eSpCas9 Protein

Description

• General description: Recombinant eSpCas9 protein from Streptococcus pyogenes (~160 KD) is a ready-to-use reagent for genome engineering experiments. When combined with target-specific guide RNAs, eSpCas9 protein will act as a targeted nuclease suitable for transfection of cell cultures and for the accelerated development of genetically-modified animals via one-cell embryo injection.
• Application: Functional Genomics/Target Validation/Genome Editing
• Features and Benefits: Enhanced specificity compared to wild type Cas9Highly activeReady-to-inject/transfect
• Packaging: pkg of 50 μgpkg of 250 μg
• Components: Each kit consists of:one vial of eSpCas9 recombinant proteinone vial containing 1 mL of 1× dilution bufferone vial containing 1 mL of nuclease-free water with glycerol
• Principle: Newly engineered eSpCas9 enables the efficient targeted gene editing of established CRISPR systems with the benefit of reduced off-target effects. Point mutations in the chromosome-binding motif of SpCas9, as described by Slaymaker, et al., provide higher on-target fidelity without loss of cleavage efficiency.[1]
• Reconstitution: Lyophilized S. pyogenes eSpCas9 protein should be resuspended in the Reconstitution solution provided to desired concentration for storage. Gently tap tube to completely dissolve lyophilized powder, incubate for 10 minutes on ice, and spin tube to bring material to bottom of tube.
• Other Notes: Use our CRISPR Selection Tool to order gRNACheck out our other MISSION® Cas9 Proteins at SigmaAldrich.com/CRISPRproteins
• Legal Information: CRISPR Use License Agreement