SHC003V
MISSION® pLKO.1-puro-CMV-TurboGFP™ Positive Control Transduction Particles
Green fluorescent protein marker to monitor transduction efficiency
Manufacturer: Sigma Aldrich
Synonym(S): MISSION®, MISSION® TurboGFP™ Control Transduction Particles
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| SHC003VN | SHC003V-SHC003VN | In Stock | ₹ 42,279.90 |
SHC003V - SHC003VN
In Stock
Quantity
1
Base Price: ₹ 42,279.90
GST (18%): ₹ 7,610.382
Total Price: ₹ 49,890.282
Quality Level
100200
product line
MISSION®
concentration
≥1x106 VP/ml (via p24 assay)
technique(s)
capture ELISA: 106 TU/mL using p24
shipped in
dry ice
storage temp.
−70°C
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Description
- General description: This construct aids in interpretation of experimental design and results by providing a green fluorescent protein marker for monitoring success in transduction of your cells of interest. TurboGFP™ is an improved variant of the green fluorescent protein copGFP cloned from the copepoda Pontellina plumata. The TurboGFP™ transduction particles are produced from the sequence-verified lentiviral plasmid, pLKO.1-puro-CMV-TurboGFP™ (SHC003). It is a positive control to monitor transduction efficiency.[1]Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the Control Transduction Particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.
- Application: MISSION® pLKO.1-puro-CMV-TurboGFP™ Positive Control Transduction Particles has been used to transduce HCT116 (human colon carcinoma) cell line for 2D culture and to form 3D spheroids. It has also been used to generate fluorescent cell lines by transduction.[2][3]
- Legal Information: MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
SAFETY INFORMATION
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Quality Level: 100200
product line: MISSION®
concentration: ≥1x106 VP/ml (via p24 assay)
technique(s): capture ELISA: 106 TU/mL using p24
shipped in: dry ice
storage temp.: −70°C
Quality Level:
100200
product line:
MISSION®
concentration:
≥1x106 VP/ml (via p24 assay)
technique(s):
capture ELISA: 106 TU/mL using p24
shipped in:
dry ice
storage temp.:
−70°C
Quality Level: 200
product line: MISSION®
concentration: 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
technique(s): __
shipped in: dry ice
storage temp.: −20°C
Quality Level:
200
product line:
MISSION®
concentration:
500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
technique(s):
__
shipped in:
dry ice
storage temp.:
−20°C
Quality Level: 200
product line: MISSION®
concentration: 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
technique(s): __
shipped in: dry ice
storage temp.: −20°C
Quality Level:
200
product line:
MISSION®
concentration:
500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
technique(s):
__
shipped in:
dry ice
storage temp.:
−20°C
Quality Level: 200
product line: MISSION®
concentration: 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
technique(s): __
shipped in: dry ice
storage temp.: −20°C
Quality Level:
200
product line:
MISSION®
concentration:
500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
technique(s):
__
shipped in:
dry ice
storage temp.:
−20°C