SHC016V
MISSION® pLKO.1-puro Non-Target shRNA Control Transduction Particles
Targets no known genes from any species
Manufacturer: Sigma Aldrich
Synonym(S): MISSION®, MISSION® Control Transduction Particles, negative control, negative shRNA control, non-target control, non-target shRNA, non-target shRNA control, shRNA control
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 EACH | SHC016V-1-EACH | In Stock | ₹ 42,279.90 |
SHC016V - 1 EACH
In Stock
Quantity
1
Base Price: ₹ 42,279.90
GST (18%): ₹ 7,610.382
Total Price: ₹ 49,890.282
Quality Level
100200
product line
MISSION®
concentration
≥1x106 VP/ml (via p24 assay)
shipped in
dry ice
storage temp.
−70°C
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Description
- General description: The MISSION® pLKO.1-puro Non-Target shRNA Control Transduction Particles contain an shRNA insert that does not target any known genes from any species, making it useful as a negative control in experiments using the MISSION® shRNA library clones.[1][2] This allows one to examine the effect of transduction of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The Non-Target shRNA Control Transduction Particles are provided as 200 μL at 1 x 106 TU/mL via p24 assay.
- Application: MISSION® pLKO.1-puro Non-Target shRNA Control Transduction Particles have been used to generate 3T3-L1 (pre-adipocytes) control cell lines.[3]
- Analysis Note: To see more application data, protocols, vector maps visit sigma.com/shrna.
- Legal Information: MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
SAFETY INFORMATION
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Quality Level: 100200
product line: MISSION®
concentration: ≥1x106 VP/ml (via p24 assay)
shipped in: dry ice
storage temp.: −70°C
Quality Level:
100200
product line:
MISSION®
concentration:
≥1x106 VP/ml (via p24 assay)
shipped in:
dry ice
storage temp.:
−70°C
Quality Level: 200
product line: MISSION®
concentration: 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
shipped in: dry ice
storage temp.: −20°C
Quality Level:
200
product line:
MISSION®
concentration:
500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
shipped in:
dry ice
storage temp.:
−20°C
Quality Level: 100200
product line: MISSION®
concentration: ≥1x106 VP/ml (via p24 assay)
shipped in: dry ice
storage temp.: −70°C
Quality Level:
100200
product line:
MISSION®
concentration:
≥1x106 VP/ml (via p24 assay)
shipped in:
dry ice
storage temp.:
−70°C
Quality Level: 200
product line: MISSION®
concentration: 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
shipped in: dry ice
storage temp.: −20°C
Quality Level:
200
product line:
MISSION®
concentration:
500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
shipped in:
dry ice
storage temp.:
−20°C