MISSION® TRC2 pLKO.5-puro Empty Vector Control Plasmid DNA

Description

• General description: The MISSION TRC2 Control Vector pLKO-puro is a lentivirus plasmid vector. This vector is in the TRC2 pLKO-puro plasmid backbone, which contains the WPRE. The vector does not contain an shRNA insert and is useful as a negative control in experiments using the TRC2 MISSION shRNA library clones. This allows one to examine the effect of transfection on gene expression and interpret the knockdown effect seen with shRNA clones.Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids (SHP001). The TRC2 pLKO-puro Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.
• Application: MISSION® TRC2 pLKO.5-puro Empty Vector Control Plasmid DNA has been used in:CRISPR library generation[1]Viral constructs[2]Plasmid construction[3]CRISPR/Cas9-mediated knockouts.[4]
• Legal Information: Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.