11373242910

Fluorescein-12-dUTP

Manufacturer: Sigma Aldrich

Synonym(S): Fluorescein-12-dUTP, dUTP

Select a Size

Pack Size SKU Availability Price
25 μL 11373242910-25-μL In Stock ₹ 28,069.23

11373242910 - 25 μL

₹ 28,069.23

In Stock

Quantity

1

Base Price: ₹ 28,069.23

GST (18%): ₹ 5,052.461

Total Price: ₹ 33,121.691

Quality Level

100

Assay

≥85% (HPLC)

form

solution

mol wt

Mr 1018.4 (fluorescein-12-dUTP-Li4)

packaging

pkg of 25 μL (25 nmol; 1mM)

manufacturer/tradename

Roche

technique(s)

ELISA: suitablehybridization: suitable

storage temp.

−20°C

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Description

  • Application: Fluorescein-12-dUTP is used as a substrate for: terminal transferaseDNA polymerase I (holoenzyme and Klenow fragment),Taq DNA polymerasereverse transcriptase (e.g., from AMV and M-MuLV).Fluorescein-12-dUTP has been used to label nuclear extracts during PCR.[1] It has also been used to replace dTTP in the random-primed DNA labeling reaction or in nick translation reactions, as well as in PCR. The nucleotide also serves as a substrate for terminal transferase in 3′-end labeling and TUNEL.[2] Fluorescein-labeled probes can be used for in in situ hybridization with direct fluorescence detection[3] or detection by enzyme linked immunoimmunsorbent assay (ELISA) using Anti-Fluorescein-AP, Fab fragments. Repeated fluorescence labeling using Tetramethyl-Rhodamine-6-dUTP (red) and AMCA-6-dUTP (bright blue) is possible.
  • Quality: Typical analysis: At least 85% Fluorescein-12-dUTP (HPLC, area%).
  • Physical form: Fluorescein-12-dUTP, tetralithium salt.
  • Storage and Stability: The unopened reagent is stable at 15 to 25°C until the expiration date printed on the label. Decomposition of approx. 5% may occur within 6 months.
  • Other Notes: For life science research only. Not for use in diagnostic procedures.

SAFETY INFORMATION

WGK

nwg

Flash Point(F)

does not flash

Flash Point(C)

does not flash

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Mr 1018.4 (fluorescein-12-dUTP-Li4)

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pkg of 25 μL (25 nmol; 1mM)

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Roche

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−20°C

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