GenElute™ Plant Genomic DNA Miniprep Kit

Description

• General description: The GenElute Plant Genomic DNA Miniprep Kit provides a simple and convenient way to isolate pure DNA from a variety of plant species. The GenElute kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, RNase treatment, and hazardous organic compounds such as phenol and chloroform.
• Application: GenElute™ Plant Genomic DNA Miniprep Kit has been used:in isolation of arbuscular mycorrhizal (AM) fungal spores DNA[1]in the purification of cetyl trimethyl ammonium bromide (CTAB)-extracted genomic DNA[2]in genomic DNA isolation from leaves[3]
• Features and Benefits: Starting material: Up to 100 mg of plant tissueExpected yield: Up to 20 μgElution volume: 100 - 200 μlTime required: < 40 minRNase treatment required: No
• Principle: Several micrograms of DNA can be obtained from up to 100 mg of fresh tissue or 10 mg of freeze-dried material in less than an hour. Plant tissue is disrupted by grinding in liquid nitrogen and the DNA is released with detergent and chaotrope. Proteins, polysaccharides, and cell debris are eliminated with a 10 minute precipitation procedure followed by centrifugation through a filtration column, included in the kit. The genomic DNA is purified further by a silica bind-wash-elute procedure in microcentrifuge spin columns. The purified DNA is greater than 20 kb in length.
• Other Notes: For additional information, please see www.sigma-aldrich.com/genomicdna.
• Legal Information: GenElute is a trademark of Sigma-Aldrich Co. LLC