GenElute™ Gel Extraction Kit

Description

• General description: The GenElute Gel Extraction Kit is designed for the rapid purification of linear and plasmid DNA fragments from standard or low-melting agarose gels. This kit can also be used to purify DNA from polyacrylamide gels. Typical recovery of DNA is up to 80%. Each column can bind up to 10 μg of DNA from up to a 3.5 g agarose slice.
• Application: GenElute™ Gel Extraction Kit has been used for the purification of DNA fragments from agarose gels.[1][2][3]
• Features and Benefits: Bind up to 10 μg of DNARecoveries up to 80%Up to 3.5 g can be processed per columnCompatible with both standard and low-melting agarose in TAE or TBE buffer
• Principle: The GenElute Gel Extraction Kit combines silica-binding technology with the convenience of a spin or vacuum column format. DNA fragments of interest are extracted by solubilizing slices of an agarose gel. The Gel Solubilization Solution dissolves an agarose gel slice from gels run in TBE or TAE buffer. The extracted DNA fragments selectively adsorb onto the silica membrane in the presence of the Gel Solubilization Solution. Contaminants are removed by a simple spin or vacuum wash. Finally, the bound DNA is eluted in Tris buffer.
• Legal Information: GenElute is a trademark of Sigma-Aldrich Co. LLC