KAPA Express Extract

Description

• General description: KAPA Express Extract is a novel thermostable protease and buffer system that allows for the extraction of polymerase chain reaction (PCR)-ready DNA in as little as 15 minutes. DNA extractions are conveniently performed in a single-tube thereby greatly reducing the risk of sample loss and contamination.The combination of KAPA Express Extract and KAPA2G Robust HotStart® ReadyMix™ provides a high performance solution for rapid DNA extraction and consistent downstream amplification.
• Application: KAPA Express Extract is ideally suited for the extraction of polymerase chain reaction (PCR)-ready DNA from, but not limited to, the following sample types:Human tissue (formalin-fixed paraffin-embedded (FFPE) samples; blood[1] collected in EDTA tubes or on collection cards; buccal swabs; hair follicles; forensic samples; biopsies)[2]Animal samples (ear punches;[3][4] tail clippings;[5][1] hair follicles; blood; bone marrow; dried or fresh tissue from mouse and other mammals)[6]Fish tissues (fin punches; fresh tissue; cold and hot smoked canned samples; ethanol-preserved samples; embryo[7])Insects (crushed)[8]Bird feathers (calamus fragments).Nosema species[9]
• Features and Benefits: Key Features:Rapid extraction protocol: PCR-ready DNA in 15 minutes.Versatility: single kit optimized for successful extraction from a variety of tissue types, including buccal swabs, hair follicles, fish tissue, and bird feathers.Single-tube reaction minimizes risk of contamination.Produces high amounts of genomic DNA of good quality.DNA extracted with KAPA Express Extract is compatible with any PCR system.Increased PCR success from formalin-fixed paraffin-embedded (FFPE) samples or tissues:Rapid DNA extraction and consistent downstream amplification of challenging sample types.Routine DNA extraction from blood sample types:Efficient extraction from “Guthrie”cards, FTA™ Elute cards, and FTA cards.Quick Notes:Extract PCR-ready DNA from different tissue types in a quick and simple, single-tube protocol (15–20 min), without the need for hazardous chemicals or multiple washing steps.Significantly reduced turnaround times and risk of sample loss and contamination.Use 1 μL DNA extract per 25 μL PCR. Depending on the sample type, the extract may have to be diluted prior to PCR.One extract typically yields a sufficient amount of template for 50–500 PCRs.DNA extracts may be diluted in TE Buffer (1:5) or 10 mM Tris-HCl pH 8–8.5 (1:5–1:10) for long-term storage at -20°C. Extracts are stable at -20°C for ≥6 months.KAPA2G Robust HotStart® ReadyMix™ is recommended for consistent and reliable endpoint amplification of DNA extracts generated with KAPA Express Extract.No quantification of DNA required prior to PCR.
• Quality: All components contained with KAPA Express Extract Kits are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activities, and meet strict requirements with respect to DNA contamination.
• Preparation Note: Handling:KAPA2G Robust HotStart ReadyMix may not freeze solidly, even when stored at -20°C. This will not affect the shelf-life of the product. Always ensure that the product has been fully thawed and mixed before use.
• Other Notes: For Research Use Only. Not for use in diagnostic procedures.
• Legal Information: FTA is a trademark of Qiagen Group