KiCqStart® SYBR® Green qPCR ReadyMix™

Description

• General description: KiCqStart SYBR Green qPCR ReadyMix is a 2X concentrated, ready-to-use master mix that contains all components, except primers and template, for real-time quantitative PCR (qPCR) This unique combination of proprietary buffer, stabilizers, and Hot-Start Taq DNA polymerase delivers maximum PCR efficiency, sensitivity, specificity and robust fluorescent signal using fast, or conventional, cycling protocols with SYBR Green qPCR.Highly specific amplification is crucial to successful qPCR with SYBR Green I dye technology because this dye binds to and detects any dsDNA generated during amplification. Hot-Start Taq DNA polymerase is antibody mediated to be inactive prior to the initial PCR denaturation step.
• Application: KiCqStart® SYBR® Green qPCR ReadyMix™ has been used:for the amplification and quantification of DNA in real-time PCR (qPCR) assay[1][2] in the amplification and quantification of transcripts in 2-step quantitative reverse transcription polymerase chain reaction (qRT-PCR)[3][4]in the amplification of complementary DNA (cDNA) by real-time PCR (qPCR) assay[5]
• Features and Benefits: Assay results in as little as 33 minutesHighly efficient and sensitive real-time PCR resultsLittle/no optimization required
• Components: 2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), KiCqStart Taq DNA Polymerase, SYBR Green dye, ROX Reference Dye (for 580-585 nm excitation), and stabilizerspackaging:250 reactions* = 2 X 1.25 mL tubes1250 reactions* = 10 X 1.25 mL tubes5000 reactions* = 1 X 50 mL tube*number of reactions based on a 20uL volume
• Quality: Kit components are free of contaminating DNase and RNase. KiCqStart® SYBR® Green qPCR ReadyMix™, Low ROX is functionally tested in qPCR. Kinetic analysis must demonstrate linear resolution over six orders of dynamic range (r2 > 0.995) and a PCR efficiency > 90%.
• Other Notes: Storage Conditions:KiCqStart SYBR Green qPCR ReadyMix is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing of the product is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20°C.
• Legal Information: 6538 is a trademark of American Type Culture Collection