JumpStart™ REDTaq® ReadyMix™ Reaction Mix

Description

• General description: JumpStart REDTaq ReadyMix PCR Reaction Mix for High Throughput PCR is formulated with REDTaq Genomic DNA polymerase for amplification of more complex templates and genomic templates. This mix contains optimized enzyme and dye concentrations to provide increased length and yield on the more difficult templates.
• Application: JumpStart™REDTaq®ReadyMix™ Reaction Mix has been used:For multiplex PCRFor amplification with reduced non-specific amplificationFor high throughput PCR amplification of complex templatesIn the amplification of primers developed for the pathogenicity-associated genes of Clavibacter michiganensis[1]For PCR amplification of the human TMPRSS6 gene[2]
• Features and Benefits: JumpStart™ REDTaq® DNA polymerase, an antibody inactivated hot start enzyme, is designed to minimize non-specific amplification while increasing target yield & specificity JumpStart Taq DNA polymerase provides superior amplification regardless of template concentration REDTaq JumpStart ReadyMix reduces pipetting steps and risk of contamination. The hot start mechanism allows for room temperature set up, making it the ideal for high throughput applications Inert red dye allows for easy verification that reagent has been mixed properlyOther loading dyes are not required. Aliquots may be directly loaded onto an agarose gel after the reaction
• Packaging: Default reaction volume is 50 μL20RXN is packaged as 1 X 500 μL100RXN is packaged as 1 X 2.5 mL800RXN is packaged as 1 X 20 mL
• Unit Definition: One unit incorporates 10 nmol of total dNTPs into acid precipitable DNA in 30 min. at 74 °C.
• Other Notes: View more detailed information on JumpStart REDTaq and ReadyMix products at www.sigma-aldrich.com/hotstart.
• Legal Information: Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.