Axygen™ AxyPrep Mag™ FragmentSelect Kits

Description

• Post DNA shearing, the library construction process for next generation sequencing mostly requires fragment selection regardless of the platform
• Obtaining high recovery post fragment selection is becoming an important contributor for the reduction of sequencing bias
• The AxyPrep FragmentSelect-I kit is optimized for Illumina next generation sequencing platforms and Life Technologies (SOLiD) fragment size selection needs, simplifying the library construction workflows
• Where, the AxyPrep FragmentSelect-R is designed for fragment size selection needs in the Roche/454s DNA Genome sequencer library construction workflow
• The AxyPrep Mag FragmentSelect kits utilize a unique paramagnetic bead technology for quick high-throughput optimized DNA size selection suitable for various next generation sequencing platforms
• Optimized fragment selection chemistries for Illumina, SOLiD and Roche/454 Versatile and flexible sequential fragment size selection: no gels needed Efficient removal of primer-dimers; no need for further clean-up steps Manual and automation friendly Scalable: Tube, 96- and 384-well microplate formats No centrifugation or filtration required AxyPrep FragmentSelect kits consists of two major stages: 1
• Optimization of AxyPrep FragmentSelect beads ratio to input DNA for desired target DNA fragment size selection 2
• The optimal AxyPrep FragmentSelect beads ratio is utilized for the isolation of the desired target DNA fragment size Note: AxyPrep FragmentSelect kits can be a one-stage process when large size DNA fragments are not a concern.