Applied Biosystems™ PrepMan™ Ultra Sample Preparation Reagent

Description

• Description The PrepMan™ Ultra Sample Preparation Reagent was developed for the rapid preparation of DNA template from Gram-negative food-borne pathogens for use in PCR amplification reactions
• These samples often have high lipid content that can inhibit PCR amplification of the template
• Using a simple boil and spin protocol, PrepMan™Ultra Reagent efficiently inactivates PCR inhibitors, significantly reducing the need to repeat the template preparation step
• PrepMan™ Ultra reagent has been used to prepare PCR-ready template from a wide variety of different sources, including bacteria, yeast, filamentous fungi, both from a plate or from liquid culture, mammalian tissue smears, human cells (buccal swab), and mammalian whole blood
• The resulting template has been used amplified in allelic discrimination assays, genetically modified organism (GMO) detection and quantification, food pathogen detection, microbial identification, and bacterial strain typing
• • Simple, rapid protocol reduces time to results
• • Unique formulation inactivates PCR inhibitors, yielding consistent results
• • PCR-ready template can be prepared from a wide range of sample types
• • Affordable method with a low price per preparation
• • Homogeneous solution with minimal sample-handling requirements
• • Microcentrifuge tube-based format means no additional equipment is needed for template preparation
• PrepMan™ Ultra reagent is a novel formulation and is a homogeneous solution that does not contain Chelex™ or any other type of resin or matrix
• This product is a replacement⁄improvement of PrepMan™ Sample Preparation Reagent, and should be recommended to current and potential PrepMan™ Reagent users
• Used With 3500/3500xL Genetic Analyzer Capillary Array (50 cm), 3130xL/3100 Genetic Analyzer Capillary Array (36 cm), 96-Well Plate Septa, AmpliTaq Gold™ PCR Master Mix, BigDye™ Terminator v1.1 Cycle Sequencing Kit, POP-6™ Polymer for 3100/3100-Avant™ Genetic Analyzers, PrepMan™ Ultra Sample Preparation Reagent, TaqMan™ GMO Maize 35S Detection Kit, TaqMan™ GMO Soy 35S Detection Kit.