Applied Biosystems™ MagMAX™ Cell-Free Total Nucleic Acid Isolation Kit

Description

• The MagMAX Cell-Free Total Nucleic Acid Isolation Kit is designed for enrichment of all nucleic acid species present in cell-free biological samples such as plasma
• The kit utilizes MagMAX magnetic-bead technology, ensuring reproducible recovery of high-quality nucleic acid that is suitable for a broad range of applications, including real-time PCR, digital PCR, and next-generation sequencing
• The magnetic bead–based purification format allows you to easily process a wide range of sample input volumes (1 mL to 6 mL)
• When used with the KingFisher Duo Prime or KingFisher Flex Magnetic Particle Processor, 6 to 24 plasma samples of 2-mL input volume can be isolated at once
• Alternatively, samples can be processed manually with the use of a magnetic stand
• Features of the MagMAX Cell-Free Total Nucleic Acid Isolation Kit include: Automation-ready, phenol-free extraction Fast procedure allows 6 to 24 samples to be processed in 90 minutes or less when used with the KingFisher Duo Prime or KingFisher Flex Magnetic Particle Processor with a 24 Deep Well Head Flexible protocol accommodates sample volume inputs ranging from 1 mL to 6 mL of plasma No need for carrier RNA Elution volumes ranging from 15 μL to 60 μL Compatible with the Oncomine Lung cfTNA Research Assay Go beyond cell-free DNA and get the total picture Isolating and analyzing circulating cell-free total nucleic acid (cfTNA) from plasma and other cell-free samples serves as a non-invasive method to detect and monitor targets associated with certain disease states such as cancer
• The first step in attaining this valuable information is efficient nucleic acid isolation that specifically recovers the circulating nucleic acid species that contains information related to the disease state
• The importance of cell-free DNA (cfDNA) is becoming established, but it may not tell the whole story
• Cell-free RNA (cfRNA) sequences serve as critical biomarkers as well in the form of microRNAs and gene fusions
• This inclusion of the cfRNA with the cfDNA provides an opportunity for researchers to expand their studies and advance human health
• The MagMAX Cell-Free Total Nucleic Acid kit is developed by the same Thermo Fisher Scientific scientists that brought you the MagMAX Cell-Free DNA Isolation Kit
• The difference is the inclusion of all cfRNA species in the eluate, allowing you to increase the amount of information available from a single cell-free sample
• Just like the cfDNA Isolation Kit, the cfTNA kit is designed for both manual and automated isolation of nucleic acid from a wide sample volume input range of 1 to 6 mL
• The phenol-free, magnetic-bead format makes the kit fully amenable to automated isolation
• Thanks to the scalable format that allows for various sample inputs, it is possible to vary the number of samples processed with a single kit
• For example, enough reagents are provided to process 50 samples of 2 mL plasma each
• Alternatively, with the same kit it is possible to process 25 samples of 4 mL plasma each
• Higher and lower input volumes are possible and the number of samples that can be processed will vary accordingly
• This unique product attribute allows you to tailor the product to your specific needs
• Benefits of MagMAX magnetic bead–based purification Magnetic beads offer many benefits compared to other technologies for isolating nucleic acid
• Beads bind the DNA more efficiently than glass-fiber filters, resulting in higher and more consistent yields
• Additionally, because filters and vacuum manifolds are not used, there is no risk of filter clogging due to cellular particulates in samples
• This clogging issue is of particular concern with protein-rich, large-volume samples such as plasma that are commonly used in circulating cell-free nucleic acid studies.