CS31103
Invitrogen™ Chargeswitch™ Pro Filter Plasmid Mini Kit
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | CS31103-Each-of-1 | In Stock | ₹ 50,138.16 |
CS31103 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 50,138.16
GST (18%): ₹ 9,024.869
Total Price: ₹ 59,163.029
Column Type
Spin Column
Format
Spin Column
Sample Type
Bacterial Culture
Elution Volume
50 to 150 μL
For Use With (Application)
Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
Includes
Miniprep Columns with Collection Tubes, Recovery Tubes, Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffers 1 and 2, and Elution Buffer
Prep Scale
< 100 μg (Small-Scale) Plasmid DNA
Product Type
Pro Filter Plasmid Mini Kit
Purity
Transfection Grade
Size
100 preps
System Type
ChargeSwitch™
Yield
30 μg
Content And Storage
The ChargeSwitch™-Pro Plasmid Miniprep Kit contains Miniprep Columns with Collection Tubes, Recovery Tubes, Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffers 1 and 2, and Elution Buffer. Store at room temperature. Guaranteed stable for 6 months when properly stored.
Isolation Technology
ChargeSwitch Chemistry
Scale
Mini
Final Product Type
Plasmid DNA
High-throughput Compatibility
Not High-throughput Compatible (Manual)
No. of Reactions
100 Preps
Product Line
ChargeSwitch™
Quantity
100 Preps
Shipping Condition
Room Temperature
Target
Plasmid DNA
Test Time
15 min.
Starting Material Amount
5 ml
Related Products
Description
- The Chargeswitch™-Pro Filter Plasmid Mini Kit includes 10 plasmid minipreps and provides high yield and high purity plasmid with the fastest and the simplest protocol
- The ChargeSwitch™-Pro Plasmid Filter Mini, Midi, Maxi Kits generates high-quality plasmid DNA without the use of harsh or potentially interfering reagents, for better success in downstream applications
- The system is based upon a novel dual-nested column design which facilitate the fastest and simplest protocol for high quality plasmid
- The system is composed of two columns
- The inner flat bottomed column, also referred to as “lysate clarification” column, contains filter materials suitable for rapid clarification of bacterial lysate
- The outer luer bottomed column, also referred to as “binding” column, contains ChargeSwitch™ (CST) derivatized membrane that immediately binds plasmid DNA from the clarified lysate
- This nested-column design enables simultaneous clarification and DNA binding in a single short centrifugation (or vacuum) step
- The combined advantages of novel, optimized plastics and a unique chemistry are an extreme competitive advantage of speed and convenience
- To accomplish this, the ChargeSwitch™-Pro Filter Kit provides: • 100% ethanol-free, guanidinium-free, organics-free, aqueous protocol for a safer, more streamlined method • Cleaner purified product for improved performance in downstream applications • A novel Dual-nested column design for simultaneous lysate clearing and binding step which offers the fastest and the simplest protocol • Endotoxin units less than 10 EU/ug DNA for • Vacuum Elution can be done with the EveryPrep™ Universal Vacuum Manifold (Cat
- No
- K211101) How it works The ChargeSwitch™ technology replaces the use of ethanol, chaotropic salts, or organic solvents for nucleic acid purification with a simple ion-exchange mechanism
- At low pH values (less than 6.5) the ChargeSwitch™ surface ligand is positively charged and binds plasmid DNA
- Raising the pH (greater than 8.5) neutralizes the charge and elutes the bound nucleic acid
- The ChargeSwitch™ protocol retains the ease and familiarity of the common silica spin column protocol with a binding, washing, and elution step, making adapting to the ChargeSwitch™ method effortless
- Order Info Shipping Condition: Room Temperature
Compare Similar Items
Show Difference
Column Type: Spin Column
Format: Spin Column
Sample Type: Bacterial Culture
Elution Volume: 50 to 150 μL
For Use With (Application): Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
Includes: Miniprep Columns with Collection Tubes, Recovery Tubes, Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffers 1 and 2, and Elution Buffer
Prep Scale: < 100 μg (Small-Scale) Plasmid DNA
Product Type: Pro Filter Plasmid Mini Kit
Purity: Transfection Grade
Size: 100 preps
System Type: ChargeSwitch™
Yield: 30 μg
Content And Storage: The ChargeSwitch™-Pro Plasmid Miniprep Kit contains Miniprep Columns with Collection Tubes, Recovery Tubes, Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffers 1 and 2, and Elution Buffer. Store at room temperature. Guaranteed stable for 6 months when properly stored.
Isolation Technology: ChargeSwitch Chemistry
Scale: Mini
Final Product Type: Plasmid DNA
High-throughput Compatibility: Not High-throughput Compatible (Manual)
No. of Reactions: 100 Preps
Product Line: ChargeSwitch™
Quantity: 100 Preps
Shipping Condition: Room Temperature
Target: Plasmid DNA
Test Time: 15 min.
Starting Material Amount: 5 ml
Column Type:
Spin Column
Format:
Spin Column
Sample Type:
Bacterial Culture
Elution Volume:
50 to 150 μL
For Use With (Application):
Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
Includes:
Miniprep Columns with Collection Tubes, Recovery Tubes, Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffers 1 and 2, and Elution Buffer
Prep Scale:
< 100 μg (Small-Scale) Plasmid DNA
Product Type:
Pro Filter Plasmid Mini Kit
Purity:
Transfection Grade
Size:
100 preps
System Type:
ChargeSwitch™
Yield:
30 μg
Content And Storage:
The ChargeSwitch™-Pro Plasmid Miniprep Kit contains Miniprep Columns with Collection Tubes, Recovery Tubes, Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffers 1 and 2, and Elution Buffer. Store at room temperature. Guaranteed stable for 6 months when properly stored.
Isolation Technology:
ChargeSwitch Chemistry
Scale:
Mini
Final Product Type:
Plasmid DNA
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
No. of Reactions:
100 Preps
Product Line:
ChargeSwitch™
Quantity:
100 Preps
Shipping Condition:
Room Temperature
Target:
Plasmid DNA
Test Time:
15 min.
Starting Material Amount:
5 ml
Column Type: Spin Column
Format: Spin Column
Sample Type: Bacterial Culture
Elution Volume: 0.5 to 1 mL
For Use With (Application): Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
Includes: Columns with Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffers 1 and 2, and Elution Buffer
Prep Scale: 100-200 μg (Medium-Scale) Plasmid DNA
Product Type: Pro Filter Plasmid Midi Kit
Purity: Transfection Grade
Size: __
System Type: ChargeSwitch™
Yield: 200 μg
Content And Storage: The ChargeSwitch™-Pro Plasmid Kit contains Columns with Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffers 1 and 2, and Elution Buffer. Store at room temperature. Guaranteed stable for 6 months when properly stored.
Isolation Technology: ChargeSwitch Chemistry
Scale: Midi
Final Product Type: Plasmid DNA
High-throughput Compatibility: Not High-throughput Compatible (Manual)
No. of Reactions: __
Product Line: ChargeSwitch™
Quantity: 25 preps
Shipping Condition: Room Temperature
Target: Plasmid DNA
Test Time: 30 min.
Starting Material Amount: 25 ml
Column Type:
Spin Column
Format:
Spin Column
Sample Type:
Bacterial Culture
Elution Volume:
0.5 to 1 mL
For Use With (Application):
Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
Includes:
Columns with Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffers 1 and 2, and Elution Buffer
Prep Scale:
100-200 μg (Medium-Scale) Plasmid DNA
Product Type:
Pro Filter Plasmid Midi Kit
Purity:
Transfection Grade
Size:
__
System Type:
ChargeSwitch™
Yield:
200 μg
Content And Storage:
The ChargeSwitch™-Pro Plasmid Kit contains Columns with Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffers 1 and 2, and Elution Buffer. Store at room temperature. Guaranteed stable for 6 months when properly stored.
Isolation Technology:
ChargeSwitch Chemistry
Scale:
Midi
Final Product Type:
Plasmid DNA
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
No. of Reactions:
__
Product Line:
ChargeSwitch™
Quantity:
25 preps
Shipping Condition:
Room Temperature
Target:
Plasmid DNA
Test Time:
30 min.
Starting Material Amount:
25 ml
Column Type: Spin Column
Format: Spin Column
Sample Type: Bacterial Culture
Elution Volume: 1 to 2 mL
For Use With (Application): Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
Includes: __
Prep Scale: > 200 μg (Large-Scale) Plasmid DNA
Product Type: Plasmid Maxi Kit
Purity: Transfection Grade
Size: __
System Type: ChargeSwitch™
Yield: 800 μg
Content And Storage: The ChargeSwitch™-Pro Plasmid Kit contains Columns with Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffers 1 and 2, and Elution Buffer. Store at room temperature. Guaranteed stable for 6 months when properly stored.
Isolation Technology: ChargeSwitch Chemistry
Scale: Maxi
Final Product Type: Plasmid DNA
High-throughput Compatibility: Not High-throughput Compatible (Manual)
No. of Reactions: 10 Preps
Product Line: ChargeSwitch™
Quantity: 10 Preps
Shipping Condition: Room Temperature
Target: Plasmid DNA
Test Time: 30 min.
Starting Material Amount: 100 ml
Column Type:
Spin Column
Format:
Spin Column
Sample Type:
Bacterial Culture
Elution Volume:
1 to 2 mL
For Use With (Application):
Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
Includes:
__
Prep Scale:
> 200 μg (Large-Scale) Plasmid DNA
Product Type:
Plasmid Maxi Kit
Purity:
Transfection Grade
Size:
__
System Type:
ChargeSwitch™
Yield:
800 μg
Content And Storage:
The ChargeSwitch™-Pro Plasmid Kit contains Columns with Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffers 1 and 2, and Elution Buffer. Store at room temperature. Guaranteed stable for 6 months when properly stored.
Isolation Technology:
ChargeSwitch Chemistry
Scale:
Maxi
Final Product Type:
Plasmid DNA
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
No. of Reactions:
10 Preps
Product Line:
ChargeSwitch™
Quantity:
10 Preps
Shipping Condition:
Room Temperature
Target:
Plasmid DNA
Test Time:
30 min.
Starting Material Amount:
100 ml
Column Type: Spin Column
Format: Spin Column
Sample Type: Bacterial Culture
Elution Volume: 1 to 2 mL
For Use With (Application): Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
Includes: __
Prep Scale: > 200 μg (Large-Scale) Plasmid DNA
Product Type: Plasmid Maxi Kit
Purity: Transfection Grade
Size: __
System Type: ChargeSwitch™
Yield: 800 μg
Content And Storage: The ChargeSwitch™-Pro Plasmid Kit contains Columns with Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffers 1 and 2, and Elution Buffer. Store at room temperature. Guaranteed stable for 6 months when properly stored.
Isolation Technology: ChargeSwitch Chemistry
Scale: Maxi
Final Product Type: Plasmid DNA
High-throughput Compatibility: Not High-throughput Compatible (Manual)
No. of Reactions: 25 Preps
Product Line: ChargeSwitch™
Quantity: 25 Preps
Shipping Condition: Room Temperature
Target: Plasmid DNA
Test Time: 30 min.
Starting Material Amount: 100 ml
Column Type:
Spin Column
Format:
Spin Column
Sample Type:
Bacterial Culture
Elution Volume:
1 to 2 mL
For Use With (Application):
Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
Includes:
__
Prep Scale:
> 200 μg (Large-Scale) Plasmid DNA
Product Type:
Plasmid Maxi Kit
Purity:
Transfection Grade
Size:
__
System Type:
ChargeSwitch™
Yield:
800 μg
Content And Storage:
The ChargeSwitch™-Pro Plasmid Kit contains Columns with Resuspension Buffer, RNase A, Lysis Buffer, Precipitation Buffer, Wash Buffers 1 and 2, and Elution Buffer. Store at room temperature. Guaranteed stable for 6 months when properly stored.
Isolation Technology:
ChargeSwitch Chemistry
Scale:
Maxi
Final Product Type:
Plasmid DNA
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
No. of Reactions:
25 Preps
Product Line:
ChargeSwitch™
Quantity:
25 Preps
Shipping Condition:
Room Temperature
Target:
Plasmid DNA
Test Time:
30 min.
Starting Material Amount:
100 ml