Thermo Scientific™ GeneJET Whole Blood Genomic DNA Purification Mini Kit

Description

• Description GeneJET Whole Blood Genomic DNA Purification Mini Kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation
• The standard procedure takes less than 20 minutes following cell lysis and yields purified DNA greater than 30 kb in size
• Isolated DNA can be used directly in PCR, Southern blotting, and enzymatic reactions
• Thermo Scientific GeneJET Whole Blood Genomic DNA Purification Mini Kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation
• The standard procedure takes less than 20 minutes following cell lysis and yields purified DNA greater than 30 kb in size
• Isolated DNA can be used directly in PCR, Southern blotting, and enzymatic reactions
• Highlights • Efficient —high yields of high molecular weight genomic DNA • Fast —20 minute procedure following cell lysis • Convenient —spin columns are capped and assembled with collection tubes • Pure —DNA pure from enzyme inhibitors, RNA and proteins
• Ready to use for all demanding applications and validated in real-time PCR, endpoint PCR, multiplex PCR, and restriction enzyme digests • Compatible —suitable for wide variety of samples like fresh or frozen anticoagulated mammalian blood (treated with EDTA, Citrate or Heparine), aged blood, buffy coat, bone marrow, and body fluids Applications • Isolated genomic DNA is ideal for use in common molecular biology procedures, including: • PCR and qPCR • FastDigest and conventional restriction digestion • Southern blotting Includes Proteinase K Solution Lysis Solution Wash Buffer WB I (concentrated) Wash Buffer II (concentrated) Elution Buffer GeneJET Purification Columns (pre-assembled with collection tubes) Collection tubes Detailed Protocol