Thermo Scientific™ GeneJET NGS Cleanup Kit

Description

• Thermo Scientific GeneJET NGS Cleanup Kit provides protocols optimized specially for fast DNA fragment cleanup or adapter removal procedures
• The clean up protocol results in higher than 70% recovery of DNA fragments larger than 150 bp and more than 97% removal of adapters
• Adapter removal protocol results in more than 70% recovery of DNA fragments larger than 200 bp and more than 99% removal of adapters
• The entire procedure takes 5 minutes for DNA fragment library cleanup from enzymatic reaction mixtures and 7 minutes for adapter removal protocol
• Spin columns technology in combination with unique binding buffer allows to get the reproducible yields of DNA fragments in the wide starting material range (5 ng - 1 μg of DNA fragments)
• The GeneJET NGS Cleanup Kit is compatible with Illumina™, Life Technologies library preparation workflows
• Highlights • Fast —high quality DNA fragments obtained within 5 min • Robust —reliable cleanup and size selection from different reaction mixtures and buffers, such as end-conversion, ligation, adapter addition, PCR mixtures, fragmentation buffers • Efficient —DNA recovery up to 90% • NGS-grade purity —free of primers, adapters, nucleotides, enzymes and other reaction components inhibiting NGS workflow
• Applications • Purified DNA fragments are ideal for use in: • PCR and qPCR • Next Generation Sequencing workflow Includes • Binding Buffer (Cat
• No
• R2811) • Prewash Buffer (conc.) (Cat
• No. R2821) • Wash Buffer (conc.)(Cat
• No. R2831) • Elution Buffer (10 mM Tris-HCl, pH 8.5) • DNA Purification Micro Column & Collection Tube • Collection Tube