Thermo Scientific™ MagJET™ngS Cleanup and Size Selection Kit

Description

• Description The MagJET NGS Cleanup and Size Selection kit is designed for efficient and robust DNA fragment library cleanup and size-selection from a variety of enzymatic reaction mixtures including PCR, ligation, adapter addition and DNA end-repair reaction mixes
• The kit utilizes high-capacity MagJET paramagnetic bead technology that ensures efficient recovery of DNA fragment library within a desired fragment length range
• The purified DNA fragments are free of any next-generation sequencing workflow inhibiting components, such as sequencing adapters, primer dimers, unincorporated nucleotides, enzymes or salts, and are ready-to-use in downstream NGS applications
• No prior DNA fragment library purification is required before the cleanup and size-selection procedures
• The kit is suitable for cleanup or size selection of 5 ng to 5 μg of DNA fragment library
• MagJET cleanup and adapter removal protocols can easily be automated for higher throughput on automatic platforms such as Thermo Scientific KingFisher Flex and Thermo Scientific KingFisher Duo
• Highlights • Robust - reliable cleanup and size selection from different reaction mixtures and buffers • Flexible - user-defined size-selection of exact DNA fragment range • NGS-grade purity - complete removal of NGS adapters, primers, nucleotides, enzymes and other reaction components inhibiting NGS workflow • High throughput compatible - easy to automate on the Thermo Scientific KingFisher magnetic particle processors for high-throughput sample handling Applications DNA fragment library cleanup and size-selection for downstream use in next generation sequencing workflows
• Includes • MagJET Magnetic Beads • Binding Solution • Wash Buffer (concentrated) • Elution Buffer • DNA Fragment Mix