Invitrogen™ PureLink™ 96 HQ Plasmid DNA Purification Kit

Description

• The PureLink HQ 96 Plasmid DNA Purification Kit is designed for high-throughput isolation of high-quality plasmid DNA from bacterial cells
• It can be used with a vacuum manifold or a centrifuge and is compatible with automated liquid handling workstations
• The PureLink HQ 96 Plasmid DNA Purification Kit offers the following advantages: • Ability to isolate up to 10 μg plasmid DNA per well • Designed to isolate high-quality plasmid DNA in 30-45 minutes • Minimal genomic DNA contamination in the purified plasmid DNA • Compatible with automated liquid handling workstations • Reliable performance of the purified plasmid DNA in downstream applications such as mammalian transfection Process overview Bacterial cells are harvested and resuspended in Resuspension Buffer with RNase
• The cells are lysed using an alkaline/SDS cell lysis procedure
• The lysate is then neutralized and conditions are adjusted for subsequent binding
• After lysate clarification using the PureLink HQ 96 Clarification Plate, the lysate is processed through the PureLink HQ 96 Binding Plate
• The DNA binds to the silica-based membrane in the plate and impurities are removed by washing with Wash Buffer
• The DNA is then eluted in low salt Elution Buffer or water
• Order Info Shipping Condition: Room Temperature