Invitrogen™ PureLink™ProQuick96 Plasmid Purification Kit

Description

• The PureLink™ Pro Quick96 Plasmid Kit combines advanced silica plate extraction chemistry with an optimized 96-well plate design for manual or automated processing of plasmid DNA from E
• coli in as little as 45 minutes (Table 1)
• Using the PureLink™ Pro Quick96 Plasmid Kit, you will: • Obtain consistently high yields of high-purity plasmid DNA • Experience ease of use and better performance due to improved plate design and protocol • Increase processing flexibility with protocols for centrifuge, vacuum manifold, or automated platforms How it works The PureLink™ Pro Quick96 Plasmid Kit uses two 96-well plates, a filtration plate (clear O-ring), and a binding plate (red O-ring) to purify plasmid DNA
• Bacterial cells are subjected to alkaline lysis and are pelleted by centrifugation
• The bacterial lysate is then applied to the Quick96 Filtration Plate for clearing and collection in the Quick96 Binding Plate, where the plasmid DNA binds to the silica membrane
• After washing steps to remove contaminants, purified DNA is eluted and ready for use in downstream applications
• The entire procedure can be completed in 45 minutes (Figure 1)
• Improved plate design The PureLink™ Pro Quick96 Plasmid Kit provides optimized semiskirted 96-well plates for improved performance and results (Figure 2)
• The Quick96 Filtration Plate is designed for fast and efficient clearing of the bacterial lysates
• The Quick96 Binding Plate offers an increased binding capacity of up to 20 μg plasmid DNA
• In addition, the plate nozzles with annular collar prevent cross-contamination of samples and improve drying of the silica membrane to prevent ethanol carryover
• In addition, sample processing can be performed manually using a benchtop vacuum manifold, by centrifugation, or with an automated liquid handling platform
• The semiskirted plate design is compatible with most vacuum manifolds on robotic workstations
• Higher yields and purity The PureLink™ Pro Quick96 Plasmid Kit allows rapid and reproducible purification of up to 20 μg of plasmid DNA from E
• coli strains grown in up to 5 mL LB, 3 mL 2xYT, or 3 mL TB
• In an automated platform using 1.5 mL E
• coli culture grown overnight in a square-well block, typical yields are 5-15 μg plasmid DNA, with an average of 9.4 μg (Figure 3)
• The resulting plasmid DNA is supercoiled with no detectable genomic DNA or RNA (Figure 4)
• The PureLink™ Pro Quick96 Plasmid Kit provides higher yields of high-quality plasmid DNA for use in common downstream applications such as automated sequencing, PCR, restriction enzyme digestion, and cloning
• Order Info Shipping Condition: Room Temperature