MP116539025
MP Biomedicals™ SPINeasy™ Plasmid Midiprep Kit
Manufacturer: MP Biomedicals™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MP116539025-Each-of-1 | In Stock | ₹ 33,690.11 |
MP116539025 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 33,690.11
GST (18%): ₹ 6,064.22
Total Price: ₹ 39,754.33
Content And Storage
Store at Room Temperature, Dry Environment
Sample Type
25 to 50 mL Bacterial (E. coli) Cultures
Quantity
25 Preps
Isolation Technology
Spin Column
Includes
60 mL Resuspension Buffer SD1, 60 mL Alkaline Lysis Buffer SD2, 90 mL Neutralization Buffer SD3, 70 mL Wash Buffer DW1, 12 mL Wash Buffer DW2, 30 mL Elution Buffer SD, 600 µL RNase A, 25 x Column SD with collection tubes
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Description
- SPINeasy Plasmid Midiprep Kit is a silica membrane spin column kit for the purification of high-copy and low-copy number plasmid DNA from 25 to 50 mL of transformed bacterial cultures
- Using our specially formulated buffers, bacterial cells are disrupted by alkaline lysis to release plasmid DNA
- Cell debris, such as chromosomal DNA and proteins, are precipitated by neutralization and cleared through centrifugation
- Subsequently, plasmid DNA in the supernatant is adsorbed on a silica membrane, washed, and eluted in a convenient process using spin column technology
- Up to 1 mg of plasmid DNA can be purified through this simple workflow, which is immediately ready for routine molecular biology applications, including cloning, in-vitro transcription, PCR, and restriction digestion.
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Content And Storage: Store at Room Temperature, Dry Environment
Sample Type: 25 to 50 mL Bacterial (E. coli) Cultures
Quantity: 25 Preps
Isolation Technology: Spin Column
Includes: 60 mL Resuspension Buffer SD1, 60 mL Alkaline Lysis Buffer SD2, 90 mL Neutralization Buffer SD3, 70 mL Wash Buffer DW1, 12 mL Wash Buffer DW2, 30 mL Elution Buffer SD, 600 µL RNase A, 25 x Column SD with collection tubes
Content And Storage:
Store at Room Temperature, Dry Environment
Sample Type:
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Spin Column
Includes:
60 mL Resuspension Buffer SD1, 60 mL Alkaline Lysis Buffer SD2, 90 mL Neutralization Buffer SD3, 70 mL Wash Buffer DW1, 12 mL Wash Buffer DW2, 30 mL Elution Buffer SD, 600 µL RNase A, 25 x Column SD with collection tubes
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