MP116545050
MP Biomedicals™ SPINeasy™ Host Depletion Microbial DNA Kit
Manufacturer: MP Biomedicals™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MP116545050-Each-of-1 | In Stock | ₹ 42,673.91 |
MP116545050 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 42,673.91
GST (18%): ₹ 7,681.304
Total Price: ₹ 50,355.214
Content And Storage
Store at Room Temperature, Dry Environment
Sample Type
Saliva, Swabs, Bodily Fluid
Quantity
50 Preps
Isolation Technology
Spin Column
Includes
12 mL Equilibration buffer,50x Lysing Matrix E, 60 mL Host Lysis Buffer, 6 mL Host Depletion Buffer, 1 Vial Host Depletion Enzyme HDE, 200 μL HDE Reconstitution Buffer, 12 mL Microbial Selection Buffer, 40 mL Microbial Lysis Buffer, 30 mL Wash Buffer HD1, 6 mL Wash Buffer HD2, 6 mL Wash Buffer HD, 50x Column HD with collection tubes
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Description
- The SPINeasy Host Depletion Microbial DNA Kit provides an easy-to-use workflow to isolate and enrich microbial DNA from samples containing high amounts of host DNA
- Host DNA contamination is common in samples such as swabs and bodily fluids
- The presence of high amounts of human DNA can limit the application and sensitivity of downstream shotgun metagenomic sequencing or microbiome analysis, as most of the extracted DNA is from human and not microbial origin
- The SPINeasy Host Depletion Microbial DNA Kit provides efficient reduction of host DNA from these samples by selective lysis of host cells with our specially formulated Host Lysis Buffer
- Host DNA, which has been released into the solution, is then removed from the supernatant through centrifugation
- A further host depletion treatment provides enzymatic degradation of any remaining host DNA, as well as DNA coming from dead microbial cells
- Finally, the remaining intact microbial cells are lysed through a combination of specially formulated buffers and highly efficient homogenization using FastPrep instruments from MP Biomedicals
- Enriched microbial DNA is then purified using a convenient silica membrane spin-column technology workflow and ready for downstream molecular applications, such as PCR and NGS.
Compare Similar Items
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Content And Storage: Store at Room Temperature, Dry Environment
Sample Type: Saliva, Swabs, Bodily Fluid
Quantity: 50 Preps
Isolation Technology: Spin Column
Includes: 12 mL Equilibration buffer,50x Lysing Matrix E, 60 mL Host Lysis Buffer, 6 mL Host Depletion Buffer, 1 Vial Host Depletion Enzyme HDE, 200 μL HDE Reconstitution Buffer, 12 mL Microbial Selection Buffer, 40 mL Microbial Lysis Buffer, 30 mL Wash Buffer HD1, 6 mL Wash Buffer HD2, 6 mL Wash Buffer HD, 50x Column HD with collection tubes
Content And Storage:
Store at Room Temperature, Dry Environment
Sample Type:
Saliva, Swabs, Bodily Fluid
Quantity:
50 Preps
Isolation Technology:
Spin Column
Includes:
12 mL Equilibration buffer,50x Lysing Matrix E, 60 mL Host Lysis Buffer, 6 mL Host Depletion Buffer, 1 Vial Host Depletion Enzyme HDE, 200 μL HDE Reconstitution Buffer, 12 mL Microbial Selection Buffer, 40 mL Microbial Lysis Buffer, 30 mL Wash Buffer HD1, 6 mL Wash Buffer HD2, 6 mL Wash Buffer HD, 50x Column HD with collection tubes
Content And Storage: Store at Room Temperature, Dry Environment
Sample Type: Up to 500 mg of Soil Samples
Quantity: 50 Preps
Isolation Technology: Spin Column
Includes: 50x Lysing Matrix YB, 2 mL RNase A Solution, 55 mL Buffer S1, 22 mL Buffer S2, 50 mL Buffer S3, 28 mL Buffer S4, 40 mL Buffer S5, 6 mL Buffer S6, 50x Column S, 50x 2 mL collection tubes, 50x 1.5 mL collection tubes
Content And Storage:
Store at Room Temperature, Dry Environment
Sample Type:
Up to 500 mg of Soil Samples
Quantity:
50 Preps
Isolation Technology:
Spin Column
Includes:
50x Lysing Matrix YB, 2 mL RNase A Solution, 55 mL Buffer S1, 22 mL Buffer S2, 50 mL Buffer S3, 28 mL Buffer S4, 40 mL Buffer S5, 6 mL Buffer S6, 50x Column S, 50x 2 mL collection tubes, 50x 1.5 mL collection tubes
Content And Storage: Store at Room Temperature, Dry Environment
Sample Type: Up to 250 mg of Stool Samples
Quantity: 50 Preps
Isolation Technology: Spin Column
Includes: 50 x Lysing Matrix YB, 45 mL Buffer SF1, 12 mL Buffer SF2, 35 mL Buffer SF3, 40 mL Buffer SF4, 40 mL Buffer SF5, 7.5 mL Buffer SF6, 50 x Column SF with collection tubes
Content And Storage:
Store at Room Temperature, Dry Environment
Sample Type:
Up to 250 mg of Stool Samples
Quantity:
50 Preps
Isolation Technology:
Spin Column
Includes:
50 x Lysing Matrix YB, 45 mL Buffer SF1, 12 mL Buffer SF2, 35 mL Buffer SF3, 40 mL Buffer SF4, 40 mL Buffer SF5, 7.5 mL Buffer SF6, 50 x Column SF with collection tubes
Content And Storage: Store at Room Temperature, Dry Environment
Sample Type: 25 to 200 µL DNA preparations > 200 bp
Quantity: 50 Preps
Isolation Technology: Spin Column
Includes: Buffer P1 50 mL, Buffer P1HA 50mL, Buffer P2 40 mL, Buffer P3 40 mL, Buffer P4 6 mL, 50x Column P, 100 x 2.0 mL collection tubes, 50 x 1.5 mL collection tubes
Content And Storage:
Store at Room Temperature, Dry Environment
Sample Type:
25 to 200 µL DNA preparations > 200 bp
Quantity:
50 Preps
Isolation Technology:
Spin Column
Includes:
Buffer P1 50 mL, Buffer P1HA 50mL, Buffer P2 40 mL, Buffer P3 40 mL, Buffer P4 6 mL, 50x Column P, 100 x 2.0 mL collection tubes, 50 x 1.5 mL collection tubes