Invitrogen™ IPTG

Description

• IPTG (isopropylthio-β-galactoside), a structural non-metabolizable analogue of allolactose, is an inducer of β-galactosidase activity in bacteria and is suitable for use with X-Gal or Bluo-Gal to detect lac gene activity in cloning procedures
• IPTG binds to the lac repressor, releasing it from the lac operator, and thereby allowing the transcription of genes in the lac operon, such as the gene coding for β-galactosidase
• In addition, IPTG is one of the most efficient molecular inducers for promoter’s transcriptional activity and is used extensively for heterologous protein expression induction in E
• coli
• Though mainly used in cell culture assays, publications demonstrated the use IPTG in vivo in animal models too
• Primary applications • Blue-white screening—induce functional β-galactosidase • Induce protein expression from inducible T7 RNA polymerase E
• coli expression systems egulated by lac operon Find the media format that fits your needs • We offer a wide variety of ready-to-use media formulations, such as LB Broth (Cat
• No
• 10855001), Terrific Broth (Cat
• No
• A1374301) or S.O.C recovery medium (Cat
• No
• 15544034)
• • X-Gal (Cat
• No
• 15520018) or Bluo-Gal (Cat
• No
• 15519028) for blue-white screening
• Order Info Approved for shipment at Room Temperature or on Wet Ice.