MilliporeSigma™ Novagen™ T7·Tag™ Affinity Purification Kit

Description

• Designed for rapid immunoaffinity purification of target proteins that carry the T7•Tag sequence Purification is based on binding target proteins to T7•Tag Monoclonal Antibody that is covalently coupled to cross-linked agarose beads, washing away unbound proteins, and eluting at pH 2.2 Neutralization Buffer is included to limit protein exposure to low pH Capacity will vary somewhat between different target proteins, but the beads are standardized to bind a minimum of 300μg T7•Tag β-galactosidase per milliliter of settled resin The beads can be used in either batch or column methods and can be regenerated a minimum of five times without loss of binding activity