Invitrogen™ BioPrime™ Total Genomic Labeling System

Description

• These complete, all-inclusive kits are optimized to work across the widest range of sample input material with no need for pre-amplification
• Highly concentrated exo-Klenow fragment of DNA polymerase I in a component limited reaction allow for consistent, robust DNA yields of ∽8μg with as little as 50ng of genomic DNA input
• An optimized dye labeled nucleotide mix with new dNTP linkers, novel, application-specific Alexa Fluor 3 and 5 dyes, and improved buffer chemistry reduce labeling variation and increase signal to noise on arrays
• Excitation and emission spectra of Alexa Fluor 3 and 5 dyes are suited for conventional two color scanners with no need to change settings
• Complete kits are batch tested to assure quality in performance from lot to lot
• Contents and Storage: The simplified kits contains Alexa Fluor 3 and Alexa Fluor 5 2x reaction mix, Exo-Klenow fragment (40U/μL), 5mM EDTA, TE Buffer, and control DNA (Salmon Sperm)
• The BioPrime Total Genomic Labeling System also contains Purelink TM Genomic DNA purification columns and purification buffers (the BioPrime Total Genomic Labeling Module does not)
• New master mix formulation streamlines reaction set-ups, increasing consistency
• Higher signal to noise with Alexa Fluor™ 3 and 5 dyes Less channel bias due to novel reaction formulation Widest range of input material (50ng - 3μg) Simplified workflow with master mix formulation Array CGH, ChIP-on-Chip, Chromatin Biology, DNA and RNA Purification and Analysis, DNA Labeling, Gene Expression Analysis and Genotyping, Genotyping and Genomic Profiling, Nucleic Acid Labeling and Oligo Synthesis, RNAi, Epigenetics and Non-Coding RNA Research