Invitrogen™ PrimeFlow™ RNA Assay Kit

Description

• This PrimeFlow™ RNA Assay is an in situ hybridization assay that combines the power of branched-DNA technology with the single-cell resolution of flow cytometry, enabling the simultaneous detection of up to four RNA targets in combination with immunophenotyping for cell surface and intracellular proteins using fluorochrome-conjugated antibodies
• Branched-DNA technology uses pairs of target-specific probes to amplify the detection of a RNA transcript, achieving between 8,000- to 16,000-fold signal amplification from a desired RNA transcript
• PrimeFlow RNA Assay can detect mRNA, long non-coding RNA, viral RNA, and telomere DNA
• The detection of microRNA is also possible and is optimal when using the PrimeFlow™ microRNA Pretreatment Buffer (cat
• 88-18006) and protocol
• PrimeFlow RNA Assay is compatible with human and mouse cells
• Other mammalian and non-mammalian cells may be compatible This PrimeFlow RNA Assay contains all the reagents needed to conduct the assay
• Positive control probes and target probes for genes of interest are sold separately
• Reported Application RNA Flow Cytometry