Invitrogen™ BioPrime™ Total FFPE Genomic Labeling System

Description

• Previous protocols have required large amounts of very high quality DNA, which is often difficult to obtain from FFPE material
• With a random prime amplification (RPA) method, using labeling mixes specifically formulated for FFPE samples, BioPrime™ Total FFPE Genomic Labeling System generates high yields of labeled DNA which is most representative of the starting sample
• Increased call accuracy is achieved with higher yields of labeled material on the array, giving you more accurate calls
• This complete, all-inclusive kit is optimized to work across a wider range of sample input material with no need for pre-amplification
• Highly concentrated exo-Klenow fragment of DNA polymerase I in a component limited reaction allow for consistent, robust DNA yields of genomic FFPE DNA input
• BioPrime™ Total for Agilent™ aCGH is supplied with Purelink™ purification which allows for low elution, eliminating the need for complicated volume-reduction steps
• Purelink™ purification also reduces background due to dye bled found in other purification systems An optimized dye labeled nucleotide mix with new dNTP linkers, novel, application-specific Alexa Fluor™ 3 and 5 dyes, and improved buffer chemistry reduce labeling variation and increase signal to noise on arrays
• Excitation and emission spectra of Alexa Fluor™ 3 and 5 dyes are suited for conventional two color scanners with no need to change settings
• Complete kits are batch tested to assure quality in performance from lot to lot
• Improved call rate with higher signal to noise ratios from Alexa Fluor™ 3 and 5 dyes Easy cleanup with PureLink™ purification eliminates the need for complicated volume-reduction steps Less channel bias due to enhanced reaction formulation for FFPE samples Lower requirements for input material using enzymatic RPA method