Invitrogen™ Click-iT™ RNA Alexa Fluor™ 488 HCS Assay

Description

• The Click-iT RNA Alexa Fluor 488 HCS Assay enables detection of global RNA transcription temporally and spatially in cells (PNAS (2008) 105:15779-84)
• The ability to detect newly synthesized RNA or changes in RNA levels resulting from disease, environmental damage or drug treatments is an important aspect of toxicological profiling
• Utilizing an alkyne-modified nucleoside, 5-ethynyl uridine (EU), and powerful click chemistry, newly synthesized RNA can be detected without the use of radioactivity or antibodies with a simple, two-step procedure
• In step one, the alkyne-containing nucleoside is fed to cells or animals and actively incorporated into nascent RNA
• The small size of the tag enables efficient incorporation of the modified nucleoside into RNA, but not into DNA
• Detection utilizes the chemoselective ligation or “click” reaction between and azide and an alkyne where the modified RNA is detected with a corresponding azide-containing dye
• With its dimunitive “footprint”, the Click-iT detection molecule can easily penetrate complex samples and leaves open the possibility of multiplex analyses with other probes, including antibodies for the detection of RNA-interactive proteins for deeper biological insights
• Less hazardous Cell Analysis, Cell Viability and Cytotoxicity, Cell Viability, Proliferation and Function, Cellular Imaging, Click Chemistries, DNA and RNA Purification and Analysis, High-Content Screening (HCS), Labeling Chemistry, Nascent RNA Detection and Capture with Click Chemistry, Nucleic Acid Labeling and Oligo Synthesis, RNA Labeling Order Info Shipping Condition: Room temperature