Invitrogen™ Quant-iT™ 1X dsDNA Assay Kits, high sensitivity (HS) and broad range (BR)

Description

• Description Make DNA quantitation easy and accurate in a ready-to-go 1X formulation with the Quant-iT 1X dsDNA HS (High-Sensitivity) and Quant-iT 1X dsDNA BR (Broad Range) Assay kits
• Both kits provide a ready-to-use working solution
• Simply load your sample, add the Qubit 1X dsDNA HS or BR working solution, and read the concentration using a fluorescence-based microplate reader
• The Quant-iT 1X dsDNA HS and BR Assay kits are highly selective for double-stranded DNA (dsDNA) over RNA, providing an accurate and precise method for the quantification of dsDNA samples
• To perform the assay, dilute your sample (any volume from 1–20 μL is acceptable) into the 1X working solution provided, then read the concentration using a fluorescence plate reader
• The Quant-iT 1X dsDNA HS Assay Kit is accurate for initial sample concentrations ranging from 10 pg/μL to 100 ng/μL, providing a core detection range of 0.2 ng to 100 ng of DNA in the assay tube
• The Quant-iT 1X dsDNA BR Assay Kit is accurate for initial DNA sample concentrations of 200 pg/μL to 4000 ng/μL (depending on sample volume), providing a detection range of 4–4000 ng of DNA
• Common contaminants such as salts, free nucleotides, solvents, detergents, and protein are well tolerated in the dsDNA assays
• The '1X' dsDNA assay kits provide reagent and buffer in a ready-to-use formulation and are highly selective for dsDNA over ssDNA, RNA, and protein.