Thermo Scientific™ ViewRNA™ HC Screening Assay

Description

• The ViewRNA HC Screening assay is an in situ hybridization method for visualization of up to 4 target RNAs, at single transcript detection level, simultaneously within individual cells
• Dual detection with protein also possible, by optimizing experimental conditions
• For flexibility, ViewRNA HC Screening Assay provided in three modules (each sold separately): ViewRNA HC Screening Assay Kit, ViewRNA HC Screening Signal Amplification Kit, and ViewRNA Probe Sets
• All there modules are needed to perform ViewRNA HC Screening Assay
• Note: ViewRNA HC Screening Assay Kit and ViewRNA HC Screening Signal Amplification Kit are made to order kits
• Please allow 4-6 weeks for shipment after placing order
• With ViewRNA Tissue assays, you can achieve Detects up to 4 RNA targets at single transcription level in cell culture cells in an automated format for 96- or 384-well plates Utilizes bright Alexa Flour 488, Alexa Flour 546, Alexa Flour 947, and/or Alexa Flour 750
• Dual detection with proteins possible, by optimizing experimental conditions
• The ViewRNA assay is a novel RNA in situ hybridization solution, based on patent-pending Probe Set design and proprietary signal amplification technology
• ViewRNA assays offers single copy RNA sensitivity in individual cells in a multiplex assay format
• Signal amplification is predicated on specific hybridization of adjacent Probe Set oligonucleotides to a target RNA, resulting in excellent signal-to-noise ratios
• Independent but compatible signal amplification systems enable simultaneous detection of up to 4 target RNAs in a single assay
• The ViewRNA HC Screening Assay is comprised of 3 modules (each sold separately): ViewRNA HC Screening Assay Kit ViewRNA HC Screening Signal Amplification Kit ViewRNA Probe Sets
• Probes can be ordered from https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cellular-imaging/in-situ-hybridization-ish/rna-fish/viewrna-cell-assays.html How it Works Step 1: Prepare Sample
• Adherent cells on a solid surface are fixed and permeabilized
• Optional perform protein detection, by following immunocytochemistry protocol
• Step 2: Hybridize Probe Sets
• Gene-specific Probe Sets hybridize to target mRNAs
• Step 3: Amplify Signal
• Independent but compatible signal amplification systems enable simultaneous detection of multiple RNAs in a single assay
• Distinct sets of PreAmp, Amp, and Label Probe molecules are used to detect different target RNAs
• Step 4: Image
• Target mRNAs are visualized using high content imaging systems or standard epifluorescence microscope
• Components of ViewRNA HC Screening Assay Kits
• Store following components at 2°C to 8°C Pretease QS: Enzyme in aqueous buffered solution Probe Set Diluent QS: Aqueous solution containing formamide and detergent Amplifier Diluent QS Aqueous solution containing formamide and detergent Label Probe Diluent QS Aqueous solution containing detergent Store following components at room temperature
• Protease Stop Buffer Aqueous buffered solution Storage Buffer Aqueous buffered solution Detergent Solution: Aqueous buffered solution containing detergent Wash Buffer Component 1 (Wash Comp 1): Aqueous solution containing detergent Wash Buffer Component 2 (Wash Comp 2): Aqueous buffered solution Pre-Hybridization Buffer (PreHyb Buffer): Aqueous buffered solution Components of ViewRNA HC Screening Signal Amplification Kits: Store following components at -20°C
• PreAmplifier (PreAmp type 4, 6, 8, or 10): DNA in aqueous buffered solution
• Amplifier (Amp type 4, 6, 8, or 10): DNA in aqueous buffered solution
• Label Probe (LP type 4-Alexa Flour 488, LP type 1-Alexa Flour 546, LP type 6-Alexa Flour 647, LP type 10-Alexa Flour 750): Fluorescent dye-conjugated oligonucleotides in aqueous buffered solution
• Note: 1-plex kits only have type 1 reagents 2-plex kits have type 1 and 6 reagents 3-plex kits have type 1, 4 and 6 reagents 4-plex kits have type 1, 4, 6 and 10 reagents