Stem cells are often defined by the expression of specific marker proteins that differ depending on cell potency and lineage
While no single marker can define a cell population, multiple markers can be used to increase the confidence and accuracy of stem Verifies differentiation state using PCR Uses multiple pluripotent and lineage-specific markers: includes 14 primers to amplify stem cell markers, 1 primer to control for cDNA synthesis, and dsDNA to serve as a positive control Efficient and cost-effect