14001012

Invitrogen™ Platinum™ II Hot-Start PCR Master Mixes (2X)

Manufacturer: Fischer Scientific

Select a Size

Pack Size SKU Availability Price
Each of 1 14001012-Each-of-1 In Stock ₹ 8,641.56

14001012 - Each of 1

₹ 8,641.56

In Stock

Quantity

1

Base Price: ₹ 8,641.56

GST (18%): ₹ 1,555.481

Total Price: ₹ 10,197.041

Content And Storage

• Platinum II Green PCR Master Mix (2X), 1.25 mL• Platinum GC Enhancer, 1.25 mL• Nuclease-free water, 1.25 mLStore at -20°C in a non-frost-free freezer.

GC-Rich PCR Performance

High

Reaction Speed

Fast or Standard

Shipping Condition

Dry Ice

Concentration

2X

Hot Start

Built-In Hot Start

Overhang

3'-A

Size (Final Product)

5 kb or less

Color

Green

Format

Tube

Polymerase

PlatinumTM II Taq Hot-Start DNA Polymerase

Product Type

Hot Start PCR Master Mix

For Use With (Application)

Hot-start PCR

Fidelity (vs. Taq)

1X

No. of Reactions

50 Reactions

Reaction Format

SuperMix or Master Mix

Starting Material

DNA

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Description

  • Invitrogen Platinum II Hot-Start PCR Master Mix (2X), available in colorless and green formats, offers Platinum II Taq Hot-Start DNA Polymerase premixed with Platinum II PCR buffer and dNTPs for convenient PCR setup
  • Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase and leading hot-start technology
  • This master mix is provided with the optional Platinum GC Enhancer for specific amplification and improved yields of GC-rich targets
  • Platinum II Hot-Start Green PCR Master Mix also includes two tracking dyes for direct loading of PCR products on gels
  • Features Innovative buffer enables universal annealing temperature by isostabilizing primer-template duplex structures Engineered Taq DNA polymerase confers fast cycling and resistance to common inhibitors Hot-start technology enables superior specificity, sensitivity, and yields and allows for room temperature reaction setup Green PCR buffer reduces pipetting errors with direct gel loading Applications Amplification of DNA from complex genomic, viral, and plasmid templates Amplification and improved yields of GC-rich targets RT-PCR Genotyping High-throughput PCR Platinum II Taq Hot-Start DNA Polymerase is an engineered Taq DNA polymerase that shows increased resistance to reaction inhibitors originating from sample material or DNA purification steps
  • The polymerase has a higher DNA synthesis rate and delivers PCR results more than two times faster than other Taq DNA polymerases
  • Proprietary Platinum Taq antibodies block polymerase activity at ambient temperatures and dissociate after the initial denaturation step at 94°C
  • This automatic hot start provides increased sensitivity, specificity, and yield, while allowing reaction assembly at room temperature
  • Due to the unique composition of the Platinum II PCR buffer, the annealing temperature is 60°C for most primer pairs designed following the general design rules
  • Isostabilizing molecules in the buffer increase primer–template duplex stability during the annealing step and contribute to enhanced specificity without the need to optimize annealing temperature for each primer pair
  • With Platinum II Hot-Start PCR Master Mix (2X), different PCR assays can be cycled together using the same protocol with universal primer annealing temperature and the extension step selected for the longest fragment to be amplified
  • Notes For applications that require PCR product analysis by absorbance or fluorescence excitation, colorless format is recommended.

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Show Difference

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Fischer Scientific

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Content And Storage:
• Platinum II Green PCR Master Mix (2X), 1.25 mL• Platinum GC Enhancer, 1.25 mL• Nuclease-free water, 1.25 mLStore at -20°C in a non-frost-free freezer.

GC-Rich PCR Performance:
High

Reaction Speed:
Fast or Standard

Shipping Condition:
Dry Ice

Concentration:
2X

Hot Start:
Built-In Hot Start

Overhang:
3'-A

Size (Final Product):
5 kb or less

Color:
Green

Format:
Tube

Polymerase:
PlatinumTM II Taq Hot-Start DNA Polymerase

Product Type:
Hot Start PCR Master Mix

For Use With (Application):
Hot-start PCR

Fidelity (vs. Taq):
1X

No. of Reactions:
50 Reactions

Reaction Format:
SuperMix or Master Mix

Starting Material:
DNA

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Fischer Scientific

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Content And Storage:
• Platinum II Green PCR Master Mix (2X), 4 x 1.25 mL• Platinum GC Enhancer, 2 x 1.25 mL• Nuclease-free water, 4 x 1.25 mLStore at -20°C in a non-frost-free freezer.

GC-Rich PCR Performance:
High

Reaction Speed:
Fast or Standard

Shipping Condition:
Dry Ice

Concentration:
2X

Hot Start:
Built-In Hot Start

Overhang:
3'-A

Size (Final Product):
5 kb or less

Color:
Green

Format:
Tube

Polymerase:
PlatinumTM II Taq Hot-Start DNA Polymerase

Product Type:
Hot Start PCR Master Mix

For Use With (Application):
Hot-start PCR

Fidelity (vs. Taq):
1X

No. of Reactions:
200 Reactions

Reaction Format:
SuperMix or Master Mix

Starting Material:
DNA

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Content And Storage:
• Platinum II Green PCR Master Mix (2X), 2 x 12.5 mL• Platinum GC Enhancer, 12.5 mL• Nuclease-free water, 2 x 12.5 mLStore at -20°C in a non-frost-free freezer.

GC-Rich PCR Performance:
High

Reaction Speed:
Fast or Standard

Shipping Condition:
Dry Ice

Concentration:
2X

Hot Start:
Built-In Hot Start

Overhang:
3'-A

Size (Final Product):
5 kb or less

Color:
Green

Format:
Tube

Polymerase:
Platinum II Taq Hot-Start DNA Polymerase

Product Type:
Hot Start PCR Master Mix

For Use With (Application):
Hot-start PCR

Fidelity (vs. Taq):
1X

No. of Reactions:
1000 Reactions

Reaction Format:
SuperMix or Master Mix

Starting Material:
DNA

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Sigma Aldrich

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Content And Storage:
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GC-Rich PCR Performance:
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Reaction Speed:
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Shipping Condition:
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Concentration:
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Hot Start:
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Overhang:
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Size (Final Product):
__

Color:
__

Format:
__

Polymerase:
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Product Type:
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For Use With (Application):
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Fidelity (vs. Taq):
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No. of Reactions:
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Reaction Format:
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Starting Material:
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