Formulated for use in one-step QRT-PCR reactions, with gene-specific primers and probes Minimal pipetting required—protocol is easy to follow and automation is viable 2X mix is supplied with a proprietary blend of RTases combining the advantages of AMV and MMuLV RTases to give unparalleled performance Can be used at elevated temperatures, avoiding problems caused by secondary structure in the RNA template QRTase Blend has greater dynamic range than either AMV or MMuLV, leading to greater accuracy in QRT-PCR experiments