43-127-04
Applied Biosystems™ SYBR™ Green Universal Master Mix
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 43-127-04-Each-of-1 | In Stock | ₹ 4,13,254.80 |
43-127-04 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 4,13,254.80
GST (18%): ₹ 74,385.864
Total Price: ₹ 4,87,640.664
Content And Storage
The 10 x 5 mL vials contain a 2X mixture of SYBR Green 1 Dye, AmpliTaq Gold™ DNA Polymerase, dNTPs with dUTP, Passive Reference 1 (ROX), and optimized buffer components. Sufficient reagents provided for 2000 reactions based on a 50 μl reaction volume.Store at -20°C.Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Format
Tube
PCR Method
qPCR
Reaction Speed
Standard
Label or Dye
SYBR Green
Product Line
SYBR™
Quantity
10 x 5 mL
For Use With (Application)
Gene Expression
No. of Reactions
2000 Reactions
Volume
10 x 5 mL
Detection Method
SYBR
GC-Rich PCR Performance
High
Polymerase
AmpliTaq Gold DNA Polymerase
For Use With (Equipment)
7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Passive Reference Dye
ROX (Pre-mixed)
Product Type
Real Time PCR SYBR Master Mix
Sufficient For
2000 Reactions of 50 μL
Concentration
2X
Sample Type
DNA (Genomic), cDNA
Related Products
Description
- Applied Biosystems™ SYBR™ Green Universal Master Mix combines SYBR™ Green I dye, AmpliTaq Gold™ DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial
- SYBR™ Green I dye detects double-stranded DNA, so specific probes are not required AmpliTaq Gold™ DNA polymerase minimizes nonspecific product formation to achieve superior performance dUTP significantly reduces carryover contamination when used in conjunction with uracil-DNA glycosylase Proprietary buffer enhancements ensure performance and reliability Premixed components stored at 2° to 8°C significantly reduce assay setup time Maximum Flexibility and Convenience: Applied Biosystems™ SYBR™ Green Universal Master Mix provides maximum flexibility at reduced cost because no target-specific TaqMan™ probes are required SYBR™ Green I dye is a double-stranded DNA binding dye that detects any double-stranded DNA generated during PCR The hot-start enzyme AmpliTaq Gold™ DNA Polymerase minimizes nonspecific product formation (including primer-dimers), yielding superior performance and sensitivity Passive Internal Reference 1 is provided to normalize non-PCR—related fluorescence fluctuations This minimizes well-to-well variability that can result from a variety of causes, such as pipetting error or sample evaporation SYBR™ Green I dye is ideal for target identification (screening assays) or when a limited number of assays is needed
- Store at -20°C Order Info Shipping Condition: Wet Ice
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Content And Storage: The 10 x 5 mL vials contain a 2X mixture of SYBR Green 1 Dye, AmpliTaq Gold™ DNA Polymerase, dNTPs with dUTP, Passive Reference 1 (ROX), and optimized buffer components. Sufficient reagents provided for 2000 reactions based on a 50 μl reaction volume.Store at -20°C.Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Format: Tube
PCR Method: qPCR
Reaction Speed: Standard
Label or Dye: SYBR Green
Product Line: SYBR™
Quantity: 10 x 5 mL
For Use With (Application): Gene Expression
No. of Reactions: 2000 Reactions
Volume: 10 x 5 mL
Detection Method: SYBR
GC-Rich PCR Performance: High
Polymerase: AmpliTaq Gold DNA Polymerase
For Use With (Equipment): 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Passive Reference Dye: ROX (Pre-mixed)
Product Type: Real Time PCR SYBR Master Mix
Sufficient For: 2000 Reactions of 50 μL
Concentration: 2X
Sample Type: DNA (Genomic), cDNA
Content And Storage:
The 10 x 5 mL vials contain a 2X mixture of SYBR Green 1 Dye, AmpliTaq Gold™ DNA Polymerase, dNTPs with dUTP, Passive Reference 1 (ROX), and optimized buffer components. Sufficient reagents provided for 2000 reactions based on a 50 μl reaction volume.Store at -20°C.Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Format:
Tube
PCR Method:
qPCR
Reaction Speed:
Standard
Label or Dye:
SYBR Green
Product Line:
SYBR™
Quantity:
10 x 5 mL
For Use With (Application):
Gene Expression
No. of Reactions:
2000 Reactions
Volume:
10 x 5 mL
Detection Method:
SYBR
GC-Rich PCR Performance:
High
Polymerase:
AmpliTaq Gold DNA Polymerase
For Use With (Equipment):
7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Passive Reference Dye:
ROX (Pre-mixed)
Product Type:
Real Time PCR SYBR Master Mix
Sufficient For:
2000 Reactions of 50 μL
Concentration:
2X
Sample Type:
DNA (Genomic), cDNA
Content And Storage: Includes: MicroAmp™ Optical Adhesive Covers (quantity 20), Applicator (quantity 1), MicroAmp™ Optical Cover Compression Pad (quantity 1)
Format: __
PCR Method: __
Reaction Speed: __
Label or Dye: __
Product Line: MicroAmp
Quantity: 1 kit
For Use With (Application): __
No. of Reactions: __
Volume: __
Detection Method: __
GC-Rich PCR Performance: __
Polymerase: __
For Use With (Equipment): 2720 Thermal Cycler, 6100 Thermal Cycler, 7000 System, 7900HT System, GeneAmp 9700, QuantStudio™, ViiA™ 7 Dx System, ViiA™ 7 System, QuantStudio™ Dx
Passive Reference Dye: __
Product Type: Film, Seal, or Applicator
Sufficient For: __
Concentration: __
Sample Type: __
Content And Storage:
Includes: MicroAmp™ Optical Adhesive Covers (quantity 20), Applicator (quantity 1), MicroAmp™ Optical Cover Compression Pad (quantity 1)
Format:
__
PCR Method:
__
Reaction Speed:
__
Label or Dye:
__
Product Line:
MicroAmp
Quantity:
1 kit
For Use With (Application):
__
No. of Reactions:
__
Volume:
__
Detection Method:
__
GC-Rich PCR Performance:
__
Polymerase:
__
For Use With (Equipment):
2720 Thermal Cycler, 6100 Thermal Cycler, 7000 System, 7900HT System, GeneAmp 9700, QuantStudio™, ViiA™ 7 Dx System, ViiA™ 7 System, QuantStudio™ Dx
Passive Reference Dye:
__
Product Type:
Film, Seal, or Applicator
Sufficient For:
__
Concentration:
__
Sample Type:
__
Content And Storage: Contains 1 MicroAmp™ Multi Removal Tool. Store at room temperature.
Format: __
PCR Method: __
Reaction Speed: __
Label or Dye: __
Product Line: MicroAmp
Quantity: 1 Tool
For Use With (Application): PCR
No. of Reactions: __
Volume: __
Detection Method: __
GC-Rich PCR Performance: __
Polymerase: __
For Use With (Equipment): Thermal Cycler, Genetic Analyzer, DNA Analyzer
Passive Reference Dye: __
Product Type: Multi Removal Tool
Sufficient For: __
Concentration: __
Sample Type: __
Content And Storage:
Contains 1 MicroAmp™ Multi Removal Tool. Store at room temperature.
Format:
__
PCR Method:
__
Reaction Speed:
__
Label or Dye:
__
Product Line:
MicroAmp
Quantity:
1 Tool
For Use With (Application):
PCR
No. of Reactions:
__
Volume:
__
Detection Method:
__
GC-Rich PCR Performance:
__
Polymerase:
__
For Use With (Equipment):
Thermal Cycler, Genetic Analyzer, DNA Analyzer
Passive Reference Dye:
__
Product Type:
Multi Removal Tool
Sufficient For:
__
Concentration:
__
Sample Type:
__
Content And Storage: Contains:5 packages of 20 plates each (equivalent to 5 × catalog # 4306737)1 package of 100 Optical Adhesive Films (equivalent to catalog # 4311971)Store at room temperature. With proper storage, shelf-life is 2 years.
Format: 96-well plate
PCR Method: __
Reaction Speed: __
Label or Dye: __
Product Line: MicroAmp
Quantity: 100 plates
For Use With (Application): __
No. of Reactions: __
Volume: __
Detection Method: __
GC-Rich PCR Performance: __
Polymerase: __
For Use With (Equipment): Thermal Cycler, Genetic Analyzer, DNA Analyzer
Passive Reference Dye: __
Product Type: Plate/Adhesive Film/Barcode
Sufficient For: __
Concentration: __
Sample Type: __
Content And Storage:
Contains:5 packages of 20 plates each (equivalent to 5 × catalog # 4306737)1 package of 100 Optical Adhesive Films (equivalent to catalog # 4311971)Store at room temperature. With proper storage, shelf-life is 2 years.
Format:
96-well plate
PCR Method:
__
Reaction Speed:
__
Label or Dye:
__
Product Line:
MicroAmp
Quantity:
100 plates
For Use With (Application):
__
No. of Reactions:
__
Volume:
__
Detection Method:
__
GC-Rich PCR Performance:
__
Polymerase:
__
For Use With (Equipment):
Thermal Cycler, Genetic Analyzer, DNA Analyzer
Passive Reference Dye:
__
Product Type:
Plate/Adhesive Film/Barcode
Sufficient For:
__
Concentration:
__
Sample Type:
__