43-988-86
Applied Biosystems™ AmpliTaq Gold™ 360 Master Mix
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 43-988-86-Each-of-1 | In Stock | ₹ 2,34,960.00 |
43-988-86 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 2,34,960.00
GST (18%): ₹ 42,292.80
Total Price: ₹ 2,77,252.80
Content And Storage
• 2X AmpliTaq Gold 360 Master Mix, 1 x 50 mL• 360 GC Enhancer, 1 x 10 mLStore at -20°C until first use, then store at 4°C.
GC-Rich PCR Performance
High
Reaction Speed
Standard
Green Features
Sustainable packaging
Quantity
50 mL
For Use With (Application)
Cloning, Hot-start PCR
Fidelity (vs. Taq)
1X
No. of Reactions
2000 Reactions
Reaction Format
SuperMix or Master Mix
Starting Material
DNA
Format
Tube
Polymerase
AmpliTaq Gold 360 DNA Polymerase
Exonuclease Activity
5' - 3'
Product Type
PCR Master Mix
Shipping Condition
Room Temp or Wet Ice
Concentration
2X
Hot Start
Built-In Hot Start
Overhang
3'-A
Size (Final Product)
5 kb or less
Volume
50 mL
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Description
- AmpliTaq Gold 360 PCR Master Mix contains everything required for successful PCR amplification in one convenient package with all components premixed and premeasured
- Designed for convenience, this master mix scales to various reaction volumes for greater application and format flexibility
- The main ingredient of the master mix is AmpliTaq Gold 360 DNA Polymerase, but it also contains the world-class 360 GC Enhancer, which can be added for high GC-content templates
- With the wide template coverage, optimization of PCR reaction conditions is virtually eliminated with the use of AmpliTaq Gold 360 Master Mix
- Features Optimized for a broad range of targets High sensitivity, specificity, and yield Robust amplification of GC-rich sequences with market-leading 360 GC Enhancer Helps achieves high-quality sequencing data Easy-to-use, premixed master mix Optimized for easy and challenging targets Challenging targets include AT-rich, GC-rich, primer-dimer forming amplicons, homopolymer repeats, and amplicons that pose sequencing challenges
- Amplicons that previously required specialized enzymes and reaction conditions can now be reproducibly amplified with a single reagent under standardized conditions
- Competitive benchmarking across more than 40 amplicons distinguishes AmpliTaq Gold 360 as a high-performing enzyme, ensuring the highest probability of success for the amplification of both everyday and challenging targets
- Optimized for automated, hot-start PCR AmpliTaq Gold 360 DNA Polymerase is the key ingredient in an automated, convenient, and efficient hot-start PCR
- When AmpliTaq Gold 360 PCR Master Mix is added to the reaction mixture at room temperature, the inactive enzyme is not capable of primer extension
- Any low-stringency mispriming events that may have occurred will not be enzymatically extended and subsequently amplified
- An initial thermal incubation step is required for activation and ensures that active enzyme is generated only at temperatures where the DNA is fully denatured
- The amount of AmpliTaq Gold 360 DNA Polymerase increases in the reaction slowly with each cycle number, and specific product yield increases without buildup of nonspecific products, including primer dimers
- The extreme specificity allows easier multiplexing and allelic discrimination
- Amplify low-copy amplicons and long targets AmpliTaq Gold 360 DNA Polymerase efficiently amplifies targets present at low copy number (see figure), even in the presence of high concentrations of complex DNA, making it especially suited for low-copy pathogen detection and amplification of targets from degraded DNA samples
- AmpliTaq Gold 360 DNA Polymerase efficiently and reproducibly amplifies long (up to 5 kb) sequences
- Notes AmpliTaq Gold 360 PCR Master Mix is supplied at 2X the recommended usage concentration for easy dilution when adding template and primers
- Order Info Approved for shipment at Room Temperature or on Wet Ice
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Content And Storage: • 2X AmpliTaq Gold 360 Master Mix, 1 x 50 mL• 360 GC Enhancer, 1 x 10 mLStore at -20°C until first use, then store at 4°C.
GC-Rich PCR Performance: High
Reaction Speed: Standard
Green Features: Sustainable packaging
Quantity: 50 mL
For Use With (Application): Cloning, Hot-start PCR
Fidelity (vs. Taq): 1X
No. of Reactions: 2000 Reactions
Reaction Format: SuperMix or Master Mix
Starting Material: DNA
Format: Tube
Polymerase: AmpliTaq Gold 360 DNA Polymerase
Exonuclease Activity: 5' - 3'
Product Type: PCR Master Mix
Shipping Condition: Room Temp or Wet Ice
Concentration: 2X
Hot Start: Built-In Hot Start
Overhang: 3'-A
Size (Final Product): 5 kb or less
Volume: 50 mL
Content And Storage:
• 2X AmpliTaq Gold 360 Master Mix, 1 x 50 mL• 360 GC Enhancer, 1 x 10 mLStore at -20°C until first use, then store at 4°C.
GC-Rich PCR Performance:
High
Reaction Speed:
Standard
Green Features:
Sustainable packaging
Quantity:
50 mL
For Use With (Application):
Cloning, Hot-start PCR
Fidelity (vs. Taq):
1X
No. of Reactions:
2000 Reactions
Reaction Format:
SuperMix or Master Mix
Starting Material:
DNA
Format:
Tube
Polymerase:
AmpliTaq Gold 360 DNA Polymerase
Exonuclease Activity:
5' - 3'
Product Type:
PCR Master Mix
Shipping Condition:
Room Temp or Wet Ice
Concentration:
2X
Hot Start:
Built-In Hot Start
Overhang:
3'-A
Size (Final Product):
5 kb or less
Volume:
50 mL
Content And Storage: • AmpliTaq Gold 360 DNA Polymerase (5 U/μL), 6 x 50 μL• 10X AmpliTaq Gold 360 Buffer, 6 x 1.5 mL• 25 mM MgCl2, 6 x 1.5 mL• 360 GC Enhancer, 6 x 1.5 mLStore at -15°C to -25°C.
GC-Rich PCR Performance: High
Reaction Speed: Standard
Green Features: Sustainable packaging
Quantity: 1500 Units
For Use With (Application): Hot-start PCR
Fidelity (vs. Taq): 1X
No. of Reactions: 1200 Reactions
Reaction Format: Separate Components
Starting Material: DNA
Format: Tube
Polymerase: AmpliTaq Gold 360 DNA Polymerase
Exonuclease Activity: 5' - 3'
Product Type: DNA Polymerase
Shipping Condition: Room Temp or Wet Ice
Concentration: 5 U/μL
Hot Start: Built-In Hot Start
Overhang: 3'-A
Size (Final Product): 5 kb or less
Volume: __
Content And Storage:
• AmpliTaq Gold 360 DNA Polymerase (5 U/μL), 6 x 50 μL• 10X AmpliTaq Gold 360 Buffer, 6 x 1.5 mL• 25 mM MgCl2, 6 x 1.5 mL• 360 GC Enhancer, 6 x 1.5 mLStore at -15°C to -25°C.
GC-Rich PCR Performance:
High
Reaction Speed:
Standard
Green Features:
Sustainable packaging
Quantity:
1500 Units
For Use With (Application):
Hot-start PCR
Fidelity (vs. Taq):
1X
No. of Reactions:
1200 Reactions
Reaction Format:
Separate Components
Starting Material:
DNA
Format:
Tube
Polymerase:
AmpliTaq Gold 360 DNA Polymerase
Exonuclease Activity:
5' - 3'
Product Type:
DNA Polymerase
Shipping Condition:
Room Temp or Wet Ice
Concentration:
5 U/μL
Hot Start:
Built-In Hot Start
Overhang:
3'-A
Size (Final Product):
5 kb or less
Volume:
__
Content And Storage: • AmpliTaq Gold 360 DNA Polymerase (5 U/μL), 12 x 50 μL• 10X AmpliTaq Gold 360 Buffer, 12 x 1.5 mL• 25 mM MgCl2, 12 x 1.5 mL• 360 GC Enhancer, 12 x 1.5 mLStore at -15°C to -25°C.
GC-Rich PCR Performance: High
Reaction Speed: Standard
Green Features: Sustainable packaging
Quantity: 3000 Units
For Use With (Application): Hot-start PCR
Fidelity (vs. Taq): 1X
No. of Reactions: 2400 Reactions
Reaction Format: Separate Components
Starting Material: DNA
Format: Tube
Polymerase: AmpliTaq Gold 360 DNA Polymerase
Exonuclease Activity: 5' - 3'
Product Type: DNA Polymerase
Shipping Condition: Room Temp or Wet Ice
Concentration: 5 U/μL
Hot Start: Built-In Hot Start
Overhang: 3'-A
Size (Final Product): 5 kb or less
Volume: __
Content And Storage:
• AmpliTaq Gold 360 DNA Polymerase (5 U/μL), 12 x 50 μL• 10X AmpliTaq Gold 360 Buffer, 12 x 1.5 mL• 25 mM MgCl2, 12 x 1.5 mL• 360 GC Enhancer, 12 x 1.5 mLStore at -15°C to -25°C.
GC-Rich PCR Performance:
High
Reaction Speed:
Standard
Green Features:
Sustainable packaging
Quantity:
3000 Units
For Use With (Application):
Hot-start PCR
Fidelity (vs. Taq):
1X
No. of Reactions:
2400 Reactions
Reaction Format:
Separate Components
Starting Material:
DNA
Format:
Tube
Polymerase:
AmpliTaq Gold 360 DNA Polymerase
Exonuclease Activity:
5' - 3'
Product Type:
DNA Polymerase
Shipping Condition:
Room Temp or Wet Ice
Concentration:
5 U/μL
Hot Start:
Built-In Hot Start
Overhang:
3'-A
Size (Final Product):
5 kb or less
Volume:
__
Content And Storage: Multiple Packs of:• AmpliTaq 360 DNA Polymerase (5 U/μL), 50 μL• 10X AmpliTaq 360 PCR Buffer, 1.5 mL• 25 mM MgCl2, 1.5 mL• 360 GC Enhancer, 1.5 mLStore at -15°C to -25°C.Product is guaranteed up to 12 months as indicated by the expiration date.
GC-Rich PCR Performance: Medium
Reaction Speed: Standard
Green Features: Sustainable packaging
Quantity: 5000 Units
For Use With (Application): Standard PCR
Fidelity (vs. Taq): 1X
No. of Reactions: 4000 Reactions
Reaction Format: Separate Components
Starting Material: DNA
Format: Stand-alone enzyme
Polymerase: AmpliTaq 360 DNA Polymerase
Exonuclease Activity: 5' - 3'
Product Type: DNA Polymerase
Shipping Condition: Room Temp or Wet Ice
Concentration: 5 U/μL
Hot Start: No
Overhang: 3'-A
Size (Final Product): 5 kb or less
Volume: __
Content And Storage:
Multiple Packs of:• AmpliTaq 360 DNA Polymerase (5 U/μL), 50 μL• 10X AmpliTaq 360 PCR Buffer, 1.5 mL• 25 mM MgCl2, 1.5 mL• 360 GC Enhancer, 1.5 mLStore at -15°C to -25°C.Product is guaranteed up to 12 months as indicated by the expiration date.
GC-Rich PCR Performance:
Medium
Reaction Speed:
Standard
Green Features:
Sustainable packaging
Quantity:
5000 Units
For Use With (Application):
Standard PCR
Fidelity (vs. Taq):
1X
No. of Reactions:
4000 Reactions
Reaction Format:
Separate Components
Starting Material:
DNA
Format:
Stand-alone enzyme
Polymerase:
AmpliTaq 360 DNA Polymerase
Exonuclease Activity:
5' - 3'
Product Type:
DNA Polymerase
Shipping Condition:
Room Temp or Wet Ice
Concentration:
5 U/μL
Hot Start:
No
Overhang:
3'-A
Size (Final Product):
5 kb or less
Volume:
__