Thermo Scientific™ Taq DNA Polymerase, recombinant (5 U/μL)

Description

• Thermo Scientific Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus
• The enzyme catalyzes 5' to 3' synthesis of DNA, has no detectable 3' to 5' exonuclease (proofreading) activity, and possesses low 5' to 3' exonuclease activity
• In addition, Taq DNA Polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products
• Recombinant Taq DNA Polymerase is the ideal tool for standard PCR of templates 5 kb or shorter
• Features Thermostable—half life is more than 40 min at 95°C Generates PCR products with 3'-dA overhangs Supplied with two buffers: 10X Taq Buffer with KCl and 10X Taq Buffer with (NH 4 ) 2 SO 4 Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescent-labeled nucleotides) Applications Routine PCR amplification of DNA fragments up to 5 kb High throughput PCR DNA labeling Notes The error rate of Taq DNA Polymerase in PCR is 2.2 x 10 -5 errors per nt per cycle
• Accordingly, the accuracy of PCR is 4.5 x 10 4
• Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs
• 10X Taq Buffer without Detergent is recommended for microarray experiments
• 10X Taq Buffer with (NH 4 ) 2 SO 4 allows for PCR at wide range of magnesium concentrations and decreases unspecific priming.