4526925
Thermo Scientific™ Verso 1-step RT-qPCR Mix, ROX
The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures.
Manufacturer: Fischer Scientific
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Detection Method
Primer-probe
GC-Rich PCR Performance
High
For Use With (Equipment)
7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
Product Type
1-Step QRT-PCR Kit w/ROX
Sample Type
RNA
Format
Tube
PCR Method
1-step RT-qPCR
Product Line
Verso
For Use With (Application)
Gene Expression, Pathogen Detection
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Description
- Thermo Scientific Verso 1-step RT-qPCR ROX Mix Kit for probe chemistry offers a quick and simple way to detect mRNA
- An optional RT Enhancer is also provided and can be used during the RT step to remove any DNA contamination, eliminating the need for DNase I treatment
- All Verso 1-step RT-qPCR kits incorporate an inert dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof
- In a 1-step approach to RT-qPCR, both the RT step and qPCR steps are performed in a single tube (1-step RT-qPCR)
- Adopting a 1-step approach is ideal when no cDNA storage is necessary for subsequent experiments
- The approach minimizes the risk of contamination and improves reproducibility by reducing sample handling
- Eliminate the need for DNase treatments - RT Enhancer (optional) prevents gDNA carry-over RT and qPCR steps performed in single tube - convenience and reduced contamination risk Use of the entire RT reaction as template for qPCR - improved sensitivity Verso RT enzyme is highly sensitive with broad dynamic range - accurate detection of RNA from 1pg to 1μg Verso RT enzyme has a wide working temperature range (42° to 57°C) - improved success with GC-rich and other difficult templates Contains an inert blue dye for ease of pipetting during reaction set up
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Detection Method: Primer-probe
GC-Rich PCR Performance: High
For Use With (Equipment): 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
Product Type: 1-Step QRT-PCR Kit w/ROX
Sample Type: RNA
Format: Tube
PCR Method: 1-step RT-qPCR
Product Line: Verso
For Use With (Application): Gene Expression, Pathogen Detection
Detection Method:
Primer-probe
GC-Rich PCR Performance:
High
For Use With (Equipment):
7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
Product Type:
1-Step QRT-PCR Kit w/ROX
Sample Type:
RNA
Format:
Tube
PCR Method:
1-step RT-qPCR
Product Line:
Verso
For Use With (Application):
Gene Expression, Pathogen Detection
Detection Method: __
GC-Rich PCR Performance: __
For Use With (Equipment): __
Product Type: __
Sample Type: __
Format: Kit
PCR Method: __
Product Line: __
For Use With (Application): RT-PCR
Detection Method:
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GC-Rich PCR Performance:
__
For Use With (Equipment):
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Product Type:
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Sample Type:
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Format:
Kit
PCR Method:
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Product Line:
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For Use With (Application):
RT-PCR
Detection Method: __
GC-Rich PCR Performance: High
For Use With (Equipment): __
Product Type: __
Sample Type: __
Format: Kit
PCR Method: __
Product Line: __
For Use With (Application): __
Detection Method:
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GC-Rich PCR Performance:
High
For Use With (Equipment):
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Product Type:
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Sample Type:
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Format:
Kit
PCR Method:
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Product Line:
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For Use With (Application):
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Detection Method: __
GC-Rich PCR Performance: High
For Use With (Equipment): __
Product Type: __
Sample Type: __
Format: Kit
PCR Method: __
Product Line: __
For Use With (Application): Real Time PCR (qPCR)
Detection Method:
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GC-Rich PCR Performance:
High
For Use With (Equipment):
__
Product Type:
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Sample Type:
__
Format:
Kit
PCR Method:
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Product Line:
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For Use With (Application):
Real Time PCR (qPCR)