Invitrogen™ Cells-to-CT™ 1-Step TaqMan™ Kit

Description

• Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step TaqMan™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification
• This new addition to the family includes a TaqMan™ 1-Step RT-PCR master mix
• The single-tube format of the RT-PCR step streamlines the 'sample to answer' workflow more than ever
• Combining the simple Cells-to- CT™ lysis technology with the fast cycling capability of the 1-step RT-PCR master mix allows quicker time to results with limited sample handling
• The Cells-to-CT™ 1-Step TaqMan™ Kit is: Complete—optimized workflow includes cell lysis reagents with gDNA removal and TaqMan™ 1-Step RT-PCR Master Mix Faster than ever—7-minute sample prep, including DNase treatment, combined with a 28-minute real-time RT-PCR step allows for ∼35 minute sample-to-answer workflow Simple—10 steps total (sample prep and real-time RT-PCR combined) Robust—perform gene expression analysis on 10–100,000 cells per sample; results equivalent to those from purified RNA Convenient—contains sufficient reagents to perform 5 real-time RT-PCRs for each cell sample you prepare; this allows you to study multiple targets or perform replicate RT-PCRs Note: TaqMan™ primer/probe sets are sold separately
• Simple 7-minute Sample Preparation, Then Ready for Real-time RT-PCR Whether you are using plates or tubes, the Cells-to-CT™ 1-Step TaqMan™ Kit uses a simple 7-minute sample preparation procedure
• The lysis technology is designed for 10–100,000 cultured cells/sample
• Cells are washed in PBS and lysed in solution for 5 minutes at room temperature with a simultaneous DNase treatment
• Lysis is terminated at room temperature by a 2-minute incubation with Stop Solution
• The lysate is now ready to analyze on your real-time PCR instrument
• Simply add it to a real-time RT-PCR master mix containing the TaqMan™ primer/probe sets of interest
• Alternatively, the lysate can be stored at –20°C
• Because samples can be processed directly in culture wells (96 or 384 wells), sample handling and the potential for sample loss or transfer error are minimized, facilitating rapid, high-throughput processing
• Unlike old-fashioned, multi-step RNA isolation protocols, no heating, washing, or centrifugation steps are required
• The kit greatly simplifies a laborious 30–60 minute process and reduces it to 7 minutes
• A Complete, Validated Solution All components of the Cells-to-CT™ 1-Step TaqMan™ Kit have been optimized for consistent and reliable performance
• This removes the guesswork involved in assembling separate sample preparation and real-time 1-step RT-PCR master mixes
• For added quality assurance, the Cells-to-CT™ 1-Step TaqMan™ Kit has been validated with TaqMan™ Gene Expression assays and shows performance equivalent to that obtained with purified RNA
• Accessory Product The TaqMan™ Cells-to-CT™ Control Kit (Cat
• No
• 4386995) was designed for use with TaqMan™ Cells-to-CT™ products
• This kit contains an endogenous control (ACTB) to normalize for sample loading variability and an artificial XenoRNA™ control and corresponding TaqMan™ Gene Expression assay to monitor the effects of PCR inhibition.