4926541
Applied Biosystems™ TaqMan™ Reverse Transcription Reagents
TaqMan Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)16, and reverse primers are included for cDNA synthesis flexibility.
Manufacturer: Fischer Scientific
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Format
Kit
Optimal Reaction Temperature
42°C
Ribonuclease H Activity
Yes
For Use With (Application)
Real Time PCR (qPCR)
Final Product Type
First-Strand cDNA
Reagent Type
Reverse Transcription
Starting Material
RNA
Technique
Reverse Transcription
Reverse Transcriptase
MultiScribe
Shipping Condition
Dry Ice
Concentration
50 U/μL
Reaction Format
Separate components
Size (Final Product)
Up to 5 kb
Related Products
Description
- TaqMan Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR)
- Random hexamers, oligo (dT) 16 , and reverse primers are included for cDNA synthesis flexibility
- These reagents are designed for reverse transcription and polymerase chain reaction amplification of a specific target RNA from either total RNA of mRNA
- The reagents in this kit facilitate two-step RT-PCR
- Two-step RT-PCR is performed in two separate reactions, useful when detecting multiple transcripts from a single cDNA reaction, or when storing a portion of the cDNA
- Advantages of TaqMan Reverse Transcription Reagents • Contains MultiScribe Reverse Transcriptase, a recombinant moloney murine leukemia virus reverse transcriptase (MMLV RT) • Random hexamers and oligo (dT) 16 included for flexibility • Individual components provide versatility in assay setup • Compatible with two-step RT-PCR reactions Using TaqMan Reverse Transcription Reagents TaqMan Reverse Transcription Reagents provide all the necessary components to perform the reverse transcription of RNA to cDNA
- For two-step RT-PCR, random hexamers, oligo (dT) 16 , and sequence-specific reverse primers can all be used
- The choice of primers for reverse transcription is best made after experimentally evaluating all three priming systems.
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Format: Kit
Optimal Reaction Temperature: 42°C
Ribonuclease H Activity: Yes
For Use With (Application): Real Time PCR (qPCR)
Final Product Type: First-Strand cDNA
Reagent Type: Reverse Transcription
Starting Material: RNA
Technique: Reverse Transcription
Reverse Transcriptase: MultiScribe
Shipping Condition: Dry Ice
Concentration: 50 U/μL
Reaction Format: Separate components
Size (Final Product): Up to 5 kb
Format:
Kit
Optimal Reaction Temperature:
42°C
Ribonuclease H Activity:
Yes
For Use With (Application):
Real Time PCR (qPCR)
Final Product Type:
First-Strand cDNA
Reagent Type:
Reverse Transcription
Starting Material:
RNA
Technique:
Reverse Transcription
Reverse Transcriptase:
MultiScribe
Shipping Condition:
Dry Ice
Concentration:
50 U/μL
Reaction Format:
Separate components
Size (Final Product):
Up to 5 kb
Format: Stand-alone Enzyme
Optimal Reaction Temperature: 37°C
Ribonuclease H Activity: Yes
For Use With (Application): __
Final Product Type: First-Strand cDNA
Reagent Type: Reverse Transcription
Starting Material: RNA
Technique: Reverse Transcription
Reverse Transcriptase: M-MLV
Shipping Condition: Wet Ice
Concentration: 200 U/μL
Reaction Format: Separate components
Size (Final Product): Up to 7 kb
Format:
Stand-alone Enzyme
Optimal Reaction Temperature:
37°C
Ribonuclease H Activity:
Yes
For Use With (Application):
__
Final Product Type:
First-Strand cDNA
Reagent Type:
Reverse Transcription
Starting Material:
RNA
Technique:
Reverse Transcription
Reverse Transcriptase:
M-MLV
Shipping Condition:
Wet Ice
Concentration:
200 U/μL
Reaction Format:
Separate components
Size (Final Product):
Up to 7 kb
Format: One-Step RT-PCR System
Optimal Reaction Temperature: 50°C
Ribonuclease H Activity: Reduced
For Use With (Application): __
Final Product Type: PCR Amplified cDNA
Reagent Type: Reverse Transcription
Starting Material: RNA
Technique: 1-Step RT-PCR
Reverse Transcriptase: SuperScript III
Shipping Condition: Dry Ice
Concentration: __
Reaction Format: Premixed Components
Size (Final Product): Up to 4.5 kb
Format:
One-Step RT-PCR System
Optimal Reaction Temperature:
50°C
Ribonuclease H Activity:
Reduced
For Use With (Application):
__
Final Product Type:
PCR Amplified cDNA
Reagent Type:
Reverse Transcription
Starting Material:
RNA
Technique:
1-Step RT-PCR
Reverse Transcriptase:
SuperScript III
Shipping Condition:
Dry Ice
Concentration:
__
Reaction Format:
Premixed Components
Size (Final Product):
Up to 4.5 kb
Format: One-Step RT-PCR System
Optimal Reaction Temperature: 50°C
Ribonuclease H Activity: Reduced
For Use With (Application): __
Final Product Type: PCR Amplified cDNA
Reagent Type: Reverse Transcription
Starting Material: RNA
Technique: 1-Step RT-PCR
Reverse Transcriptase: SuperScript III
Shipping Condition: Dry Ice
Concentration: __
Reaction Format: Premixed Components
Size (Final Product): Up to 10 kb
Format:
One-Step RT-PCR System
Optimal Reaction Temperature:
50°C
Ribonuclease H Activity:
Reduced
For Use With (Application):
__
Final Product Type:
PCR Amplified cDNA
Reagent Type:
Reverse Transcription
Starting Material:
RNA
Technique:
1-Step RT-PCR
Reverse Transcriptase:
SuperScript III
Shipping Condition:
Dry Ice
Concentration:
__
Reaction Format:
Premixed Components
Size (Final Product):
Up to 10 kb