Invitrogen™ SYBR GreenER™ qPCR SuperMix Universal

Description

• SYBR™ GreenER™ qPCR SuperMixes Universal incorporate the latest high-performance technology to provide the most reliable gene expression data on a variety of platforms, including ABI 7500, Corbett Rotor-Gene™, MJ Chromo4™ and Opticon™, and Stratagene instruments
• The SYBR™ GreenER™ system is specially formulated to offer the best sensitivity, specificity, and reproducibility with
• A novel dsDNA-binding dye exhibits a brighter signal for increased sensitivity and less PCR inhibition than original SYBR™ Green I, while using the same instrument filters and settings
• An optimized buffer system improves sensitivity and provides excellent long-term stability
• Uracil DNA glycosylase reduces carryover contamination in qPCR
• Eight cDNA targets and eight genomic DNA targets were amplified from 1ng of cDNA and 6pg of gDNA, respectively, on the ABI PRISM™ 7900HT Sequence Detection System using the SYBR™ GreenER™ qPCR SuperMix for ABI PRISM™ instruments
• For all 16 targets, detection was positive in the presence of template but negative for all no-template controls
• The SYBR™ GreenER™ qPCR SuperMix kits include a new dsDNA-binding dye that produces a brighter signal and significantly less PCR inhibition, for improved qPCR performance over a broad dynamic range Consistent Specificity and Low-Copy Detection: The SYBR™ GreenER™ qPCR reagent system minimizes the formation of nonspecific products, including primer-dimers, for greater accuracy across a wide range of targets The reliability of the SYBR™ GreenER™ qPCR reagent system also translates to greater sensitivity With SYBR™ GreenER™ qPCR SuperMix, you can consistently detect fewer than 10 copies of a target in genomic DNA