Applied Biosystems™ Custom TaqMan™ Gene Expression Assay, VIC

Description

• Applied Biosystems Custom TaqMan Gene Expression assays, VIC, are the custom version of our predesigned TaqMan assays for quantitative real-time PCR
• Custom TaqMan Gene Expression assays consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (VIC) on the 5’ end and a minor groove binder (MGB) and nonfluorescent quencher (NFQ) on the 3’ end
• Features include: • Easy to use —just add cDNA and master mix and run the qPCR—no melt curves required • Specific —TaqMan assays use proprietary MGB-containing probes that can be up to 15 bases shorter than non-MGB probes, improving the specificity of the assay • Sensitive —TaqMan assays are ideal for measuring low levels of expression or low-abundance targets • Accurate —identify small fold-changes with high accuracy of quantitation • Gold-standard TaqMan qPCR chemistry —TaqMan assays draw on Thermo Fisher Scientific's bioinformatics assay design pipeline to help ensure high specificity and minimal cross-reactivity, even for gene variants with high sequence homology • Ideal for multiplexing —combine one FAM-labeled and one VIC-labeled assay to create a duplex assay for two different gene targets in the same qPCR well • Sequences provided —with every Custom TaqMan Gene Expression you design and order Approximate ship time Made to order: 4–6 days in North America, 6–10 days in Europe Pre-formulated assay • 2 unlabeled PCR primers (900 nM each final 1X concentration) • 1 VIC dye-labeled TaqMan MGB probe (250 nM final 1X concentration) TaqMan Gene Expression assays are the gold standard in real-time PCR gene expression studies, built on more than 20 years of experience
• Each assay includes target primers and a sequence-specific probe optimized for the best functional performance
• No additional design, optimization, or melt curve analysis is needed
• Available in a wide variety of formats and species, new assay designs are constantly added to help meet your research needs
• TaqMan assays have been cited in over 40,000 publications and are backed by more than 350 patents.