7181282

Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents with manual

AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Description

Works on all AB platforms, but is optimized for the 7500.

Format

Tube

PCR Method

1-step RT-qPCR

Reaction Speed

Standard

Passive Reference Dye

ROX (Pre-mixed)

Product Type

One-Step RT-PCR Reagent

Concentration

25X

Detection Method

Primer-probe

GC-Rich PCR Performance

High

Polymerase

AmpliTaq Gold DNA Polymerase

For Use With (Equipment)

7500 System

Product Line

AgPath-ID™, Ambion™

Reverse Transcriptase

ArrayScript™

Fidelity (vs. Taq)

25 X

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Description

  • Recommended for RNA pathogen amplification Consistent amplification of RNA targets with high specificity and sensitivity Optimized to work with your target-specific primers and probes Contains ROX for quantitative fluorescent signal normalization AgPath-ID™ One-Step RT-PCR Reagents are configured for fast and simple reaction setup
  • The reactions are assembled in a single tube, minimizing sample handling errors and expediting set-up time
  • Once assembled, results are available in approximately one hour
  • The 25X RT-PCR Enzyme Mix included in the kit contains highly efficient ArrayScript™ Reverse Transcriptase, a mutant MMLV RT that produces high cDNA yields, and AmpliTaq Gold™ polymerase, the preferred hot-start DNA polymerase for specific target amplification
  • The 2X RT-PCR Buffer has been optimized for efficient, robust reverse transcription and PCR includes the passive reference dye, ROX™ Dye, for quantitative fluorescent signal normalization.

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7181282

AgPath-ID™ One-Step RT-PCR Reagents...


Description:
Works on all AB platforms, but is optimized for the 7500.

Format:
Tube

PCR Method:
1-step RT-qPCR

Reaction Speed:
Standard

Passive Reference Dye:
ROX (Pre-mixed)

Product Type:
One-Step RT-PCR Reagent

Concentration:
25X

Detection Method:
Primer-probe

GC-Rich PCR Performance:
High

Polymerase:
AmpliTaq Gold DNA Polymerase

For Use With (Equipment):
7500 System

Product Line:
AgPath-ID™, Ambion™

Reverse Transcriptase:
ArrayScript™

Fidelity (vs. Taq):
25 X

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