Applied Biosystems™ resDNASEQ™ QuantitativeE. coliDNA Kits

Description

• This quantitative PCR (qPCR)-based kit, part of the resDNASEQ™ E
• coli Residual DNA Quantitation System, enables highly sensitive quantitation of host cell DNA from E
• coli expression systems, typically in less than 4 hours
• The kit is part of an integrated system that includes sample preparation, TaqMan™ Assay and master mix, standard DNA, instrument, and software
• The system overcomes the limitations of traditional methods by combining high-recovery PrepSEQ™ sample preparation and TaqMan™ Assay-based quantitation of residual DNA
• Features of the resDNASEQ Quantitative E
• coli DNA Kit include: • Highly sensitive quantitation using proven TaqMan™ real-time qPCR technology • Manual and automated sample preparation, optimized for quantitative recovery from complex sample matrices (Table 1) • Consistent performance across the expected range of DNA fragment sizes • Integrated system from sample to results with sample preparation, master mix, TaqMan™ primer/probe mix, and genomic DNA standard This kit is available with or without sample preparation reagents in the form of a PrepSEQ™ Residual DNA Sample Preparation Kit, which is optimized for highly efficient residual DNA recovery from complex mixtures of proteins, buffers, and salts
• Rapid, Sensitive Detection of E.Coli DNA The resDNASEQ™ E
• coli Residual DNA Quantitation System is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from E
• coli , an expression system commonly used for the production of recombinant proteins
• Reliable and rapid, the system enables sensitive (LOQ = 15 pg DNA/mL test sample) and specific quantitation of E
• coli DNA, typically in less than 4 hours
• This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types—from in-process samples to bulk drug substances—whether a sample contains high molecular weight or sheared DNA
• Table 1
• DNA recovery using manual PrepSEQ™ sample preparation protocol
• Assay performance data using 10 pg E
• coli genomic DNA spike per sample, 3 analysts, and 9 test samples
• 50 mg/mL protein sample Mean Recovery Mean %CV E
• coli 83% 5.04%