A21178

Invitrogen™ GeneArt™ CRISPR Nuclease Vector with OFP Reporter Kit (with competent cells)

Manufacturer: Invitrogen™

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Pack Size SKU Availability Price
Each of 1 A21178-Each-of-1 In Stock ₹ 84,533.28

A21178 - Each of 1

₹ 84,533.28

In Stock

Quantity

1

Base Price: ₹ 84,533.28

GST (18%): ₹ 15,215.99

Total Price: ₹ 99,749.27

Cloning Method

Restriction Enzyme/MCS

Detection Method

Primer-probe

Format

Kit

Technique

CRISPR-Cas9

Product Type

Crispr Nuclease Vector With ofP Reporter Kit

Protein Tag

OFP fusion

Sufficient For

10 Reactions

No. of Reactions

10 Reactions

Starting Material

Oligos (DNA)

Content And Storage

• CRISPR OFP Nuclease Vector, linearized• 10X Oligonucleotide Annealing Buffer• DNase/RNase-free Water• 5X Ligation Buffer• T4 DNA Ligase• U6 Forward Sequencing Primer• Control, double stranded oligonucleotideStore the above at -5 to -30°C.• OneShot™ TOP10 Chemically Competent CellsStore at -80°C.

Form

Liquid

Reaction Speed

Fast

Product Line

GeneArt™

Promoter

U6, CMV

Reporter Gene

OFP

For Use With (Application)

Gene Expression, Cloning

Overhang

3' overhangs

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Description

  • Simple, ready-to-use, all-in-one expression vector system for expression of the functional components needed for CRISPR-Cas9 genome editing in mammalian cells with an orange fluorescent protein (OFP) reporter
  • GeneArt CRISPR Nuclease Vector with OFP Reporter Kit is a vector system for expression of the functional components needed for CRISPR-Cas9 genome editing in mammalian cells with an orange fluorescent protein (OFP) reporter
  • The OFP reporter allows for fluorescence-based tracking of transfection efficiency, as well as FACS-based sorting/enrichment of Cas9 and CRISPR expressing cells
  • The linearized GeneArt™ CRISPR nuclease vectors provide a rapid and efficient way to clone double-stranded oligonucleotides encoding a crRNA representing a desired target into an expression cassette that allows sequence-specific targeting of the Cas9 nuclease
  • Competent cells included for added convenience and highest cloning efficiencies
  • GeneArt™ CRISPR Nuclease Vector system offers: Easy-to-design genome engineering system Affordable, ready-to-use cloning vectors Enrichment for hard to transfect or difficult to modify cell For research use only
  • Not for use in diagnostic procedures
  • Certifications CoA Compliance CoA Recommended Storage Store in freezer (-5 to -30°C)

Compare Similar Items

Show Difference

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Invitrogen™

A21178

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Cloning Method:
Restriction Enzyme/MCS

Detection Method:
Primer-probe

Format:
Kit

Technique:
CRISPR-Cas9

Product Type:
Crispr Nuclease Vector With ofP Reporter Kit

Protein Tag:
OFP fusion

Sufficient For:
10 Reactions

No. of Reactions:
10 Reactions

Starting Material:
Oligos (DNA)

Content And Storage:
• CRISPR OFP Nuclease Vector, linearized• 10X Oligonucleotide Annealing Buffer• DNase/RNase-free Water• 5X Ligation Buffer• T4 DNA Ligase• U6 Forward Sequencing Primer• Control, double stranded oligonucleotideStore the above at -5 to -30°C.• OneShot™ TOP10 Chemically Competent CellsStore at -80°C.

Form:
Liquid

Reaction Speed:
Fast

Product Line:
GeneArt™

Promoter:
U6, CMV

Reporter Gene:
OFP

For Use With (Application):
Gene Expression, Cloning

Overhang:
3' overhangs

Img

Sigma Aldrich

A211850

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Cloning Method:
__

Detection Method:
__

Format:
__

Technique:
__

Product Type:
__

Protein Tag:
__

Sufficient For:
__

No. of Reactions:
__

Starting Material:
__

Content And Storage:
__

Form:
__

Reaction Speed:
__

Product Line:
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Promoter:
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Reporter Gene:
__

For Use With (Application):
__

Overhang:
__

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Invitrogen

A21253

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Cloning Method:
__

Detection Method:
__

Format:
__

Technique:
__

Product Type:
Antibody

Protein Tag:
__

Sufficient For:
__

No. of Reactions:
__

Starting Material:
__

Content And Storage:
-20°C

Form:
Lyophilized

Reaction Speed:
__

Product Line:
__

Promoter:
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Reporter Gene:
__

For Use With (Application):
__

Overhang:
__

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Invitrogen

A21271

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Cloning Method:
__

Detection Method:
__

Format:
__

Technique:
__

Product Type:
Antibody

Protein Tag:
__

Sufficient For:
__

No. of Reactions:
__

Starting Material:
__

Content And Storage:
-20°C

Form:
Lyophilized

Reaction Speed:
__

Product Line:
__

Promoter:
__

Reporter Gene:
__

For Use With (Application):
__

Overhang:
__