A25918
Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | A25918-Each-of-1 | In Stock | ₹ 82,993.20 |
A25918 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 82,993.20
GST (18%): ₹ 14,938.776
Total Price: ₹ 97,931.976
Content And Storage
The 2X mix contains SYBR Green dye, Dual-Lock Taq DNA Polymerase, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye, and optimized buffer components. Contains 10 x 1 mL tube, sufficient for 1000 20-μL reactions (10 μL Master Mix per reaction).Store at 2–8°C in the dark.
Format
Tube
Polymerase
Dual-Lock Taq DNA Polymerase
For Use With (Equipment)
7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 7 Flex, StepOne™, StepOnePlus™, ViiA™ 7 System
Product Type
Real Time PCR SYBR Master Mix
Shipping Condition
Wet Ice
For Use With (Application)
Gene Expression
No. of Reactions
1000 Reactions
Volume
10 x 1 mL
Detection Method
SYBR
PCR Method
qPCR
Reaction Speed
Fast or Standard
Product Line
PowerUP™, SYBR™
Quantity
10 x 1 mL
Sufficient For
1000 Reactions of 20 μL
Concentration
2X
Sample Type
DNA
Related Products
Description
- Coupled with user-supplied primer sets and template, PowerUp™ SYBR™ Green Master Mix is designed to amplify targets for accurate gene expression analysis
- A dual hot-start mechanism for excellent specificity Highly reproducible CTs over a broad dynamic range Inclusion of UDG to help prevent carryover contamination Stability of pre-assembled reactions for up to 72 hours Compatibility with most real-time qPCR instruments Exceptional specificity with dual hot-start mechanism: Specificity is paramount in obtaining high-quality data in SYBR™ Green reactions and can be enhanced through control of the Taq DNA polymerase at lower temperatures before stringent primer binding PowerUp™ SYBR™ Green Master Mix uses the Dual-Lock™ Taq DNA polymerase enzyme that combines two unique hot-start mechanisms to help control its activity and to prevent undesirable early activity of the polymerase at low temperatures In an experiment evaluating 24 different primer sets, PowerUp™ SYBR™ Green Master Mix generated single melt curves 100% of the time, consistent with highly specific amplification Tight reproducibility in CTs over a wide dynamic range: PowerUp™ SYBR™ Green Master Mix demonstrates excellent reproducibility over a wide dynamic range for a variety of targets tested In addition, this master mix provides efficient amplification over 6 logs of sample input Unlike competitor mixes that can exhibit inhibition at high cDNA inputs and low correlation coefficients over a dynamic range experiment, PowerUp™ SYBR™ Green Master Mix efficiency is maintained over the full dynamic range to help deliver maximal confidence in data quality UDG included for carryover contamination control: The inclusion of uracil-DNA glycosylase (UDG) and dUTP in the PowerUp™ SYBR™ Green Master Mix enables the degradation of any previously amplified PCR products, helping prevent contamination of subsequent qPCR reactions and possible false positives Stability of pre-assembled reactions: The tight control of the Dual-Lock™ Taq DNA polymerase in PowerUp™ SYBR™ Green Master Mix enables reactions to be assembled up to 72 hours prior to cycling without impacting data This consistency allows users to have confidence and flexibility in the use of PowerUp™ SYBR™ Green Master Mix across numerous workflow scenarios1 Broad instrument compatibility: PowerUp™ SYBR™ Green Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems™ real-time PCR instruments2 It is also compatible with the Bio-Rad IQ™5 and CFX96™/CFX384™ systems, Roche LightCycler™ LC480, and Stratagene™ MX3005P™ systems For optimal results, recommended primer concentrations are 300 to 800nM Order Info Shipping Conditions: Wet Ice
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Content And Storage: The 2X mix contains SYBR Green dye, Dual-Lock Taq DNA Polymerase, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye, and optimized buffer components. Contains 10 x 1 mL tube, sufficient for 1000 20-μL reactions (10 μL Master Mix per reaction).Store at 2–8°C in the dark.
Format: Tube
Polymerase: Dual-Lock Taq DNA Polymerase
For Use With (Equipment): 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 7 Flex, StepOne™, StepOnePlus™, ViiA™ 7 System
Product Type: Real Time PCR SYBR Master Mix
Shipping Condition: Wet Ice
For Use With (Application): Gene Expression
No. of Reactions: 1000 Reactions
Volume: 10 x 1 mL
Detection Method: SYBR
PCR Method: qPCR
Reaction Speed: Fast or Standard
Product Line: PowerUP™, SYBR™
Quantity: 10 x 1 mL
Sufficient For: 1000 Reactions of 20 μL
Concentration: 2X
Sample Type: DNA
Content And Storage:
The 2X mix contains SYBR Green dye, Dual-Lock Taq DNA Polymerase, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye, and optimized buffer components. Contains 10 x 1 mL tube, sufficient for 1000 20-μL reactions (10 μL Master Mix per reaction).Store at 2–8°C in the dark.
Format:
Tube
Polymerase:
Dual-Lock Taq DNA Polymerase
For Use With (Equipment):
7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 7 Flex, StepOne™, StepOnePlus™, ViiA™ 7 System
Product Type:
Real Time PCR SYBR Master Mix
Shipping Condition:
Wet Ice
For Use With (Application):
Gene Expression
No. of Reactions:
1000 Reactions
Volume:
10 x 1 mL
Detection Method:
SYBR
PCR Method:
qPCR
Reaction Speed:
Fast or Standard
Product Line:
PowerUP™, SYBR™
Quantity:
10 x 1 mL
Sufficient For:
1000 Reactions of 20 μL
Concentration:
2X
Sample Type:
DNA
Content And Storage: • DNA Binding Beads, 2 x 8 mL (store at 2°C to 8°C, DO NOT FREEZE)• DNA Elution Buffer 1, 1 x 25 mL (store at room temperature)• DNA Elution Buffer 2, 1 x 25 mL (store at room temperature)• Wash Solution 1 Concentrate, 1 x 80 mL* (store at room temperature)• Wash Solution 2 Concentrate, 1 x 162 mL* (store at room temperature)• Multi-Sample DNA Lysis Buffer, 1 x 100 mL (store at room temperature)• Nuclease-Free Water, 1 x 100 mL (store at room temperature)• PK Buffer, 1 x 96 mL (store at room temperature)• Proteinase K, 2 x 4 mL (store at -5° to -30°C)• Ribonuclease A (RNase A), 1 x 1.25 mL (store at -5° to -30°C)*after addition of alcohol
Format: Kit
Polymerase: __
For Use With (Equipment): __
Product Type: DNA Multi-Sample Ultra Kit
Shipping Condition: __
For Use With (Application): Next-Generation Sequencing, Real-Time Quantitative PCR (qPCR)
No. of Reactions: 500 Preps
Volume: __
Detection Method: __
PCR Method: __
Reaction Speed: __
Product Line: MagMAX™
Quantity: 500 Preps
Sufficient For: __
Concentration: __
Sample Type: Blood, Buccal Samples, Cells, Liquid Samples (e.g. Serum), Tissue
Content And Storage:
• DNA Binding Beads, 2 x 8 mL (store at 2°C to 8°C, DO NOT FREEZE)• DNA Elution Buffer 1, 1 x 25 mL (store at room temperature)• DNA Elution Buffer 2, 1 x 25 mL (store at room temperature)• Wash Solution 1 Concentrate, 1 x 80 mL* (store at room temperature)• Wash Solution 2 Concentrate, 1 x 162 mL* (store at room temperature)• Multi-Sample DNA Lysis Buffer, 1 x 100 mL (store at room temperature)• Nuclease-Free Water, 1 x 100 mL (store at room temperature)• PK Buffer, 1 x 96 mL (store at room temperature)• Proteinase K, 2 x 4 mL (store at -5° to -30°C)• Ribonuclease A (RNase A), 1 x 1.25 mL (store at -5° to -30°C)*after addition of alcohol
Format:
Kit
Polymerase:
__
For Use With (Equipment):
__
Product Type:
DNA Multi-Sample Ultra Kit
Shipping Condition:
__
For Use With (Application):
Next-Generation Sequencing, Real-Time Quantitative PCR (qPCR)
No. of Reactions:
500 Preps
Volume:
__
Detection Method:
__
PCR Method:
__
Reaction Speed:
__
Product Line:
MagMAX™
Quantity:
500 Preps
Sufficient For:
__
Concentration:
__
Sample Type:
Blood, Buccal Samples, Cells, Liquid Samples (e.g. Serum), Tissue
Content And Storage: • DNA Binding Beads, 10 x 8 mL (store at 2°C to 8°C, DO NOT FREEZE)• DNA Elution Buffer 1, 5 x 25 mL (store at room temperature)• DNA Elution Buffer 2, 5 x 25 mL (store at room temperature)• Wash Solution 1 Concentrate, 5 x 80 mL* (store at room temperature)• Wash Solution 2 Concentrate, 5 x 162 mL* (store at room temperature)• Multi-Sample DNA Lysis Buffer, 5 x 100 mL (store at room temperature)• Nuclease-Free Water, 5 x 100 mL (store at room temperature)• PK Buffer, 5 x 96 mL (store at room temperature)• Proteinase K, 10 x 4 mL (store at -5° to -30°C)• Ribonuclease A (RNase A), 5 x 1.25 mL (store at -5° to -30°C)*after addition of alcohol
Format: Kit
Polymerase: __
For Use With (Equipment): __
Product Type: DNA Multi-Sample Ultra Kit
Shipping Condition: __
For Use With (Application): Next-Generation Sequencing, Real-Time Quantitative PCR (qPCR)
No. of Reactions: 2500 Preps
Volume: __
Detection Method: __
PCR Method: __
Reaction Speed: __
Product Line: MagMAX™
Quantity: 2500 Preps
Sufficient For: __
Concentration: 1 ng/μL to 3 ng/μL
Sample Type: Blood, Buccal Samples, Cells, Liquid Samples (e.g. Serum), Tissue
Content And Storage:
• DNA Binding Beads, 10 x 8 mL (store at 2°C to 8°C, DO NOT FREEZE)• DNA Elution Buffer 1, 5 x 25 mL (store at room temperature)• DNA Elution Buffer 2, 5 x 25 mL (store at room temperature)• Wash Solution 1 Concentrate, 5 x 80 mL* (store at room temperature)• Wash Solution 2 Concentrate, 5 x 162 mL* (store at room temperature)• Multi-Sample DNA Lysis Buffer, 5 x 100 mL (store at room temperature)• Nuclease-Free Water, 5 x 100 mL (store at room temperature)• PK Buffer, 5 x 96 mL (store at room temperature)• Proteinase K, 10 x 4 mL (store at -5° to -30°C)• Ribonuclease A (RNase A), 5 x 1.25 mL (store at -5° to -30°C)*after addition of alcohol
Format:
Kit
Polymerase:
__
For Use With (Equipment):
__
Product Type:
DNA Multi-Sample Ultra Kit
Shipping Condition:
__
For Use With (Application):
Next-Generation Sequencing, Real-Time Quantitative PCR (qPCR)
No. of Reactions:
2500 Preps
Volume:
__
Detection Method:
__
PCR Method:
__
Reaction Speed:
__
Product Line:
MagMAX™
Quantity:
2500 Preps
Sufficient For:
__
Concentration:
1 ng/μL to 3 ng/μL
Sample Type:
Blood, Buccal Samples, Cells, Liquid Samples (e.g. Serum), Tissue
Content And Storage: • 100 μg rhLaminin-521, store at -10 to -30°C, protect from light• 500 mL basal medium, store at 2-8°C, protect from light• 10 mL supplement, store at -5 to -20°C, protect from light
Format: __
Polymerase: __
For Use With (Equipment): __
Product Type: Stem Cell Adaptation Kit
Shipping Condition: Supplement and matrix - dry ice, Basal - ambient
For Use With (Application): __
No. of Reactions: __
Volume: __
Detection Method: __
PCR Method: __
Reaction Speed: __
Product Line: Essential 8™
Quantity: 1 kit
Sufficient For: __
Concentration: __
Sample Type: __
Content And Storage:
• 100 μg rhLaminin-521, store at -10 to -30°C, protect from light• 500 mL basal medium, store at 2-8°C, protect from light• 10 mL supplement, store at -5 to -20°C, protect from light
Format:
__
Polymerase:
__
For Use With (Equipment):
__
Product Type:
Stem Cell Adaptation Kit
Shipping Condition:
Supplement and matrix - dry ice, Basal - ambient
For Use With (Application):
__
No. of Reactions:
__
Volume:
__
Detection Method:
__
PCR Method:
__
Reaction Speed:
__
Product Line:
Essential 8™
Quantity:
1 kit
Sufficient For:
__
Concentration:
__
Sample Type:
__