Applied Biosystems™ TaqMan™ Arbovirus Triplex Kit (ZIKV/DENV/CHIKV), 0.1 mL

Description

• The TaqMan Arbovirus Triplex Kit enables the simultaneous detection and differentiation of RNA from dengue virus (DENV), chikungunya virus (CHIKV), and Zika virus (ZIKV) in urine and serum
• It is a lyophilized, multiplex, one-step real-time PCR Applied Biosystems TaqMan assay for the molecular detection of dengue, chikungunya, and Zika RNA
• The kit is optimized for use with the Applied Biosystems QuantStudio 5 Real-Time PCR System, but is also suitable for the 7500, 7500 Fast, and other QuantStudio systems
• It includes a primer-probe set for human PPIA (cyclophilin A) as an endogenous extraction, RT-PCR and PCR amplification control
• The kit is compatible with RNA extracted from human urine or serum using the MagMAX Pathogen RNA/DNA Kit
• The Zika virus sequence used to develop these primers and probes is from a 2014 research paper by Pyke et al (1)
• Features of the kit: • Lyophilized with one-step RT-PCR master mix; simply add RNA • Simple workflow for detection of Zika, Dengue, and Chikungunya virus RNA from urine or serum • Easy to use and environmentally friendly
• No cold chain management required; shipped and stored at room temperature
• Contents: • Twelve 8-well strips tubes for 96 total reactions per kit • 0.1-mL tube format, compatible with 96-well fast block • Primer-probe detection with 4 probe reporter dyes: --FAM-Zika --VIC-Dengue --ABY-Chikungunya --JUN-PPIA cyclophilin 1
• Pyke AT, Daly MT, Cameron JN, Moore PR, Taylor CT, Hewitson GR, Humphreys JL, Gair R
• Imported zika virus infection from the cook islands into australia, 2014
• PLoS Curr
• 2014 Jun 2;6
• Order Info Ambient Temperature