Applied Biosystems™ Detection Enhancer, for real-time PCR

Description

• Detection Enhancer is uniquely formulated to improve template amplification in real-time PCR (qPCR) workflows
• This reagent is particularly beneficial with DNA samples containing GC-rich sequences and/or persistent secondary structure
• Detection Enhancer is a component of the AgPath-ID One-Step RT-qPCR Reagents kit and is offered here as a stand-alone product that can be used with any Applied Biosystem real-time PCR master mix
• Improve qPCR amplification of templates containing high GC content and persistent secondary structure Compatible with any Applied Biosystem real-time PCR master mix Flexible kit sizes for a range of laboratory throughputs GC-rich DNA sequences are usually defined as those that contain >60% cytosine (C) or guanine (G)
• GC-rich samples can be particularly challenging to amplify because the sequences are more stable than those with lower GC content
• In qPCR experiments, this results in a higher DNA melting point
• In addition, GC-rich sequences can form secondary structure, which can add further amplification challenges
• Adding a small volume of Detection Enhancer to your reaction mix can dramatically improve qPCR amplification in GC-rich samples
• Detection Enhancer is specifically formulated to address qPCR amplification in particularly stable DNA sequences
• It is only for use in real-time PCR workflows where amplification is affected by templates that are GC rich and/or contain persistent secondary structure
• The reagents may affect sensitivity for other target types, so it is recommended to first assemble the reaction without Detection Enhancer
• If no signal is observed from samples that are expected to be positive, then add Detection Enhancer to the reaction
• The recommended volume of Detection Enhancer per 25 μL reaction is 1.67 μL
• For more information on applications of Detection Enhancer, please see the AgPath-ID One-Step RT-qPCR Reagents kit user guide