Applied Biosystems™ PowerTrack™ SYBR Green Master Mix for qPCR

Description

• Coupled with user-supplied primer sets and template, PowerTrack SYBR Green Master Mix is designed to amplifiy targets for accurate gene expression analysis
• Features of PowerTrack SYBR Green Master Mix include: • Built-in visual indicators aid in reaction setup • Broad primer Tm and concentration compatibility allows qPCR reaction setup flexibility with minimal optimization • Specific and tight reproducibility in Ct’s over a broad dynamic range • Formulated with UNG/dUTP to prevent contamination of carryover PCR products Built-in visual indicators PowerTrack SYBR Green Master Mix contains an inert blue dye that does not interfere with real-time PCR but provides increased visibility in the tube or well
• A separate sample buffer is provided that contains an inert yellow dye
• The optional use of this yellow sample buffer results in a reaction mixture that changes from blue to green upon addition of the sample, helping one avoid pipetting mistakes
• The yellow sample buffer aids in reaction setup, but is not required to obtain superior results with PowerTrack SYBR Green Master Mix
• Formulated for maximum specificity and reproducibility PowerTrack SYBR Green Master Mix contains Taq DNA polymerase, which is tightly controlled through an antibody-mediated hot-start mechanism, helping prevent undesirable early activity of the polymerase at low temperatures that can lead to nonspecific amplification
• PowerTrack SYBR Green Master Mix is designed for exceptional performance in your most challenging real-time PCR applications
• Broad instrument compatibility PowerTrack SYBR Green Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems real-time PCR instruments
• It is also compatible with the Bio-Rad CFX96™, CFX384™, and iQ™5; Roche LightCycler™ 480; and Agilent Mx3005P™ systems
• For optimal results, primer concentrations should be in the range of 300–800 nM
• UNG for carryover contamination control Contamination in labs that routinely run PCR is a major concern for many researchers and is the source of most false positives
• The inclusion of UNG and dUTP in PowerTrack SYBR Green Master Mix results in the degradation of any previously amplified PCR products and helps prevent contamination of subsequent qPCR reactions.