SureTect™ Listeria species PCR Assay

Description

• Detect Listeria species simply, reliably and rapidly in even the most challenging food types with the Thermo Scientific™ SureTect™ Listeria Species PCR Assay, which incorporates optimized primer and probe components for highly-specific detection of Listeria species
• The assay's workflow holds AOAC Performance Tested Methods SM approval granted by the AOAC Research Institute and is an AOAC Official Method SM , with First Action status granted by AOAC International
• The assay is also validated according to ISO 16140-2:2016 and certified by NF Validation™ by AFNOR Certification
• The SureTect System is an optimized solution combining speed and performance in an easy-to-use, cost-effective platform—giving you results you can trust
• The new SureTect assay format incorporates color-coded plastics with orientation markings and breakable strip format, colored reagents, and pierceable seals to enable an optimized workflow for maximum laboratory efficiency and productivity
• Simple Easy-to-use with single enrichment step and pre-dispensed reagents Target-specific colored assay plastics Clearly oriented lysis and assay trays Color indicator in lysis reagent reducing possibility for human error Fast and Flexible Option to automate the lysis and PCR sample preparation Optimized workflow delivers on-screen results in as little as 22 hours Maximum flexibility for operational efficiency Run all assays simultaneously in the same run Test up to 96 samples in each run Choice of pierceable seals or caps Detect multiple targets in the same run with universal PCR protocols across the range Smart Intuitive, user-friendly software delivers reliable results, with no subjective interpretation Actionable data supports fast decisions in a complex product supply chain IPC in every assay for complete confidence in results The SureTect Listeria species PCR Assay Kit includes: Lysis Reagent 1 Tubes Lysis Reagent 2 Proteinase K Listeria Species PCR Assay Tubes Thermo Scientific 96-Well Pierceable Seals PCR Caps Enriched samples are pipetted into pre-filled Lysis tubes, along with Proteinase K and Lysis Reagent 2, before incubation to lyse any bacteria cells present in the sample and release their DNA into the solution
• The lysate is then loaded into the SureTect Listeria Species PCR tube to re-hydrate the preloaded PCR pellet, which contains all the necessary components and reagents for PCR, including a probe, primers and DNA template for the internal positive control (IPC)
• The SureTect Listeria Species PCR Assay is validated on the Applied Biosystems™ QuantStudio™ 5 Food Safety Real-Time PCR System used with RapidFinder™ Analysis Software v3.0 (or later) or the Applied Biosystems™ 7500 Fast Food Safety 96-well Real-Time PCR instrument used with RapidFinder™ Express Software v2.0 or later
• After completing the run, interpreted results are clearly displayed and can be reported, stored, printed or downloaded as required.