AB4104C
Thermo Scientific™ Verso 1-step RT-qPCR Kit, SYBR Green, with separate ROX vial
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | AB4104C-Each-of-1 | In Stock | ₹ 1,17,046.08 |
AB4104C - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,17,046.08
GST (18%): ₹ 21,068.294
Total Price: ₹ 1,38,114.374
Content And Storage
Contains:• 1 x 50 μL Verso Enzyme Mix• 1 x 250 μL RT enhancer• 2 x 1.25 mL 2X 1-Step qPCR SYBR Mix• 1 x 25 μL ROX, 1mM• 1 x 100 μL 1M MgCl2Sufficient for 200 x 25 μL reactions.Store at -20°C
Format
Tube
PCR Method
1-step RT-qPCR
Product Line
Verso
Sufficient For
400 Reactions
No. of Reactions
400 Reactions
Detection Method
SYBR
GC-Rich PCR Performance
High
For Use With (Equipment)
BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
Product Type
1-Step RT-qPCR Kit
For Use With (Application)
Gene Expression, Pathogen Detection
Sample Type
RNA
Related Products
Description
- The Thermo Scientific Verso 1-step RT-qPCR Kit for SYBR Green chemistry offers a quick and simple way to detect mRNA
- This version of the kit comes with a separate vial of ROX reference dye
- An optional RT Enhancer is also provided and can be used during the RT step to remove any DNA contamination, eliminating the need for DNase I treatment
- All Verso 1-step RT-qPCR kits incorporate an inert dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof
- In a 1-step approach to RT-qPCR, both the RT step and qPCR steps are performed in a single tube (1-step RT-qPCR)
- Adopting a 1-step approach is ideal for primer sequence optimization and collecting comparative data in experiments that do not require subsequent cDNA storage
- The approach minimizes the risk of contamination, and improvements in reproducibility may be observed as a result of reduced sample handling
- Verso One-Step RT-qPCR Kits for SYBR Green chemistry are compatible with all major real-time thermal cyclers
- Eliminate the need for DNase treatments - RT Enhancer (optional) prevents gDNA carry-over RT and qPCR steps performed in single tube - convenience and reduced contamination risk Use of the entire RT reaction as template for qPCR - improved sensitivity Verso RT enzyme is highly sensitive with broad dynamic range - accurate detection of RNA from 1pg to 1μg Verso RT enzyme has a wide working temperature range (42° to 57°C) - improved success with GC-rich and other difficult templates Contains an inert blue dye for ease of pipetting during reaction setup
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Content And Storage: Contains:• 1 x 50 μL Verso Enzyme Mix• 1 x 250 μL RT enhancer• 2 x 1.25 mL 2X 1-Step qPCR SYBR Mix• 1 x 25 μL ROX, 1mM• 1 x 100 μL 1M MgCl2Sufficient for 200 x 25 μL reactions.Store at -20°C
Format: Tube
PCR Method: 1-step RT-qPCR
Product Line: Verso
Sufficient For: 400 Reactions
No. of Reactions: 400 Reactions
Detection Method: SYBR
GC-Rich PCR Performance: High
For Use With (Equipment): BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
Product Type: 1-Step RT-qPCR Kit
For Use With (Application): Gene Expression, Pathogen Detection
Sample Type: RNA
Content And Storage:
Contains:• 1 x 50 μL Verso Enzyme Mix• 1 x 250 μL RT enhancer• 2 x 1.25 mL 2X 1-Step qPCR SYBR Mix• 1 x 25 μL ROX, 1mM• 1 x 100 μL 1M MgCl2Sufficient for 200 x 25 μL reactions.Store at -20°C
Format:
Tube
PCR Method:
1-step RT-qPCR
Product Line:
Verso
Sufficient For:
400 Reactions
No. of Reactions:
400 Reactions
Detection Method:
SYBR
GC-Rich PCR Performance:
High
For Use With (Equipment):
BioRad CFX96™, MJ Chromo4, MJ Opticon, Eppendorf MasterCycler RealPlex, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, Corbett RotorGene
Product Type:
1-Step RT-qPCR Kit
For Use With (Application):
Gene Expression, Pathogen Detection
Sample Type:
RNA
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