Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents

Description

• Recommended for RNA pathogen amplification Consistent amplification of RNA targets with high specificity and sensitivity Optimized to work with your target-specific primers and probes Contains ROX for quantitative fluorescent signal normalization AgPath-ID™ One-Step RT-PCR Reagents are configured for fast and simple reaction setup
• The reactions are assembled in a single tube, minimizing sample handling errors and expediting set-up time
• Once assembled, results are available in approximately one hour
• The 25X RT-PCR Enzyme Mix included in the kit contains highly efficient ArrayScript™ Reverse Transcriptase, a mutant MMLV RT that produces high cDNA yields, and AmpliTaq Gold™ polymerase, the preferred hot-start DNA polymerase for specific target amplification
• The 2X RT-PCR Buffer has been optimized for efficient, robust reverse transcription and PCR includes the passive reference dye, ROX™ Dye, for quantitative fluorescent signal normalization
• A Detection Enhancer is also provided as an optional reagent for amplification of templates with a high GC content or persistent secondary structure.